Gill ATPase activities of silver perch, Bidyanus bidyanus (Mitchell), and golden perch, Macquaria ambigua (Richardson): Effects of environmental salt and ammonia

• Published in: Aquaculture Vol. 251; no. 1; pp. 118 - 133
• Main Authors: Alam, Mostafa, Frankel, Theresa L.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 20.01.2006; Elsevier Science; Elsevier Sequoia S.A
• Subjects: Adenosine triphosphatase; Agnatha. Pisces; Ammonia; Animal aquaculture; Animal productions; Aquaculture; ATPase; Bidyanus bidyanus; Biological and medical sciences; Fish; Freshwater; Freshwater teleosts; Fundamental and applied biological sciences. Psychology; General aspects; Macquaria ambigua; Salt; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution; Australia; Salt; Ammonia; ATPase; Freshwater teleosts; Gill; Enzyme; Adenosinetriphosphatase; Freshwater environment; Vertebrata; Salt effect; Pisces; Hydrolases; Teleostei; Osteichthyes; Aquaculture
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2005.05.028

The Australian freshwater fish, silver and golden perch, are increasingly being used for aquaculture. Addition of salt to water is commonly used in commercial aquaculture to reduce stress attributed to high ammonia concentrations. The activities in gill homogenates of ouabain-sensitive Na +/K +-ATPase and NEM-sensitive ATPases (as a measure of H +-ATPases) of silver and golden perch were measured after maintaining the fish in water containing different salt and ammonia concentrations. Six treatments were applied in a 2 × 3 factorial design: two salt treatments, low salt (LS) of 2.5 g l − 1 and high salt (HS) 5 g l − 1 , and three ammonia treatments, no added ammonia (NA), low ammonia (LA), 3 mg total ammonia nitrogen (TAN) l − 1 and high ammonia (HA), 5 mg TAN l − 1 . In both species, activity of Na +/K +-ATPase was lowest in fish kept in the LSNA treatment (7.4 ± 0.4 μmol Pi mg protein − 1 h − 1 for silver perch and 3.1 ± 0.6 for golden perch) and highest in the HSHA treatment (15.2 ± 1.0 μmol Pi mg − 1 protein h − 1 for silver and 8.4 ± 1.2 for golden perch). In both species there was a significant increase ( P < 0.001) in Na +/K +-ATPase activity with increase in salt concentration and with an increase in ammonia concentrations. A significant interaction ( P < 0.036) between salt and ammonia on Na +/K +-ATPase activity was observed in silver but not in golden perch. In contrast, the lowest activity for NEM-sensitive ATPase was observed in the HSNA treatment (1.0 ± 0.2 μmol Pi mg − 1 protein h − 1 for silver and 1.5 ± 0.4 for golden perch) and highest in LSHA treatment (2.9 ± 0.4 μmol Pi mg − 1 protein h − 1 for silver and 3.6 ± 1.2 for golden perch). In both species there was a significant decrease in NEM-sensitive ATPase activity with increase in salt concentration and an increase in activity with increase in ammonia ( P < 0.003). In silver perch, a significant interaction between the treatments was observed ( P < 0.02). The results suggest that in these species of freshwater fish the Na +/K +-ATPase has a role in salt and ammonia homeostasis and that the NEM-sensitive ATPases are more active in fish kept in water with a lower salt content. It is possible that the increase in ammonia resistance when salt is added to the environmental water in commercial aquaculture systems may be due to the effects of salt on gill Na +/K +-ATPase activity rather than the NEM-sensitive ATPases.