Anti-inflammatory, antioxidant, anti-tyrosinase and phenolic contents of four Podocarpus species used in traditional medicine in South Africa
Leaf and stem extracts of four species of South African Podocarpus species were evaluated for their anti-inflammatory, antioxidant and tyrosinase inhibitory activities. Their phenolic content was also determined. The four species exhibited significant anti-inflammatory, antioxidant and anti-tyrosina...
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Published in | Journal of ethnopharmacology Vol. 136; no. 3; pp. 496 - 503 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Ireland
Elsevier Ireland Ltd
14.07.2011
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Subjects | |
Online Access | Get full text |
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Summary: | Leaf and stem extracts of four species of South African
Podocarpus species were evaluated for their anti-inflammatory, antioxidant and tyrosinase inhibitory activities. Their phenolic content was also determined. The four species exhibited significant anti-inflammatory, antioxidant and anti-tyrosinase.
Species of
Podocarpus are used traditionally in their native areas for the treatment of fevers, asthma, coughs, cholera, chest complaints, arthritis, rheumatism, venereal diseases and distemper in dogs.
To investigate the antioxidant, anti-inflammatory and anti-tyrosinase activities of four
Podocarpus species,
Podocarpus elongatus,
Podocarpus falcatus,
Podocarpus henkelii and
Podocarpus latifolius, used in traditional medicine in South Africa. Phytochemical analysis to determine the phenolic contents was also carried out.
DPPH, FRAP and β-carotene-linoleic acid assays were used to determine the antioxidant/radical scavenging activities of these species. Anti-inflammatory activity of these species was assayed against two cyclooxygenase enzymes (COX-1 and COX-2). Tyrosinase inhibition activity was analysed using the modified dopachrome method with
l-DOPA as the substrate. Phenolics were quantitatively determined using spectrophotometric methods.
Stems of
Podocarpus latifolius exhibited the lowest EC
50 (0.84
μg/ml) inhibition against DPPH. The percentage antioxidant activity based on the bleaching rate of β-carotene ranged from 96% to 99%. High ferric reducing power was observed in all the extracts. For COX-1, the lowest EC
50 value was exhibited by stem extracts of
Podocarpus elongatus (5.02
μg/ml) and leaf extract of
Podocarpus latifolius showed the lowest EC
50 against COX-2 (5.13
μg/ml). All extracts inhibited tyrosinase activity in a dose-dependent manner with stem extract of
Podocarpus elongatus being the most potent with an EC
50 value of 0.14
mg/ml. The total phenolic content ranged from 2.38 to 6.94
mg of GAE/g dry sample.
The significant pharmacological activities observed support the use of these species in traditional medicine and may also be candidates in the search for modern pharmaceuticals in medicine, food and cosmetic industries. |
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Bibliography: | http://dx.doi.org/10.1016/j.jep.2010.07.019 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0378-8741 1872-7573 |
DOI: | 10.1016/j.jep.2010.07.019 |