Genome-wide transcriptional response of the Arctic bacterium Pseudoalteromonas sp. A2 to oxidative stress induced by hydrogen peroxide

• Published in: Acta oceanologica Sinica Vol. 35; no. 12; pp. 73 - 80
• Main Authors: Lin, Xuezheng, Wang, Zhen, Li, Yang, Li, Jiang
• Format: Journal Article
• Language: English
• Published: Beijing The Chinese Society of Oceanography 01.12.2016; Springer Nature B.V; Key Laboratory of Marine Bioactive Substances, the First Institute of 0ceanography, State 0ceanic Administration, Qingdao 266061, China
• Subjects: Adaptation; Amino acids; Bacteria; Chaperones; Chemical analysis; Climatology; Cytochrome; Cytochromes; Down-regulation; Earth and Environmental Science; Earth Sciences; Ecology; Engineering Fluid Dynamics; Environmental Chemistry; Flagella; Gene expression; Gene regulation; Genes; Genomes; Heat shock; Heat shock proteins; Hydrogen; Hydrogen peroxide; Low temperature; Marine; Marine & Freshwater Sciences; Metabolism; Oceanography; Oxidative stress; Protein turnover; Proteins; Pseudoalteromonas; Receptors; Seawater; Stress proteins; Transcriptomes; Water analysis; Arctic region; PN, Arctic; Arctic; RNA-Seq; oxidative stress; Pseudoalteromonas
• ISSN: 0253-505X; 1869-1099
• DOI: 10.1007/s13131-016-0892-z

Oxidative stress is one of the major challenges faced by Arctic marine bacteria due to the high oxygen concentration of seawater, low temperatures and UV radiations. Transcriptome sequencing was performed to obtain the key functional genes involved in the adaptation to oxidative stress induced by hydrogen peroxide in the Arctic bacterium Pseudoalteromonas sp. A2. Exposure to 1 mmol/L H 2 O 2 resulted in large alterations of the transcriptome profile, including significant up-regulation of 109 genes and significant down-regulation of 174 genes. COG functional classification revealed that among the significantly regulated genes with known function categories, more genes belonging to posttranslational modification, protein turnover and chaperones were significantly up-regulated, and more genes affiliated with chaperones and amino acid transport and metabolism were significantly down-regulated. It was notable that the expressions of eighteen genes affiliated with flagella and four genes affiliated with heat shock proteins were significantly up-regulated. Meanwhile, the expression of nine genes belonging to cytochrome and cytochrome oxidase, and five genes belonging to TonB-dependent receptor, were significantly down-regulated. Among the eighteen genes with antioxidant activity categorized by GO analysis, the expression of one gene was significantly up-regulated; however, the expressions of two genes were significantly down-regulated. Briefly, RNA-Seq indicated that, except for the classical anti-oxidative genes and stress proteins, genes affiliated with flagella and function unknown played important roles in coping with oxidative stress in Pseudoalteromonas sp. A2. This overall survey of transcriptome and oxidative stress-relevant genes can contribute to understand the adaptive mechanism of Arctic bacteria.