Movement protein-derived resistance to triple gene block-containing plant viruses

• Published in: Journal of general virology Vol. 78; no. 6; pp. 1241 - 1246
• Main Authors: Seppanen, P, Puska, R, Honkanen, J, Tyulkina, LG, Fedorkin, O, Morozov, SYu, Atabekov, JG
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01.06.1997
• Subjects: Amino Acid Sequence; Molecular Sequence Data; Plant Viral Movement Proteins; Plant Viruses - genetics; Plants, Genetically Modified; RNA, Viral - physiology; Viral Proteins - physiology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-78-6-1241

P Seppanen, R Puska, J Honkanen, LG Tyulkina, O Fedorkin, SYu Morozov and JG Atabekov Department of Biological Research, Kemira Agro OY, Espoo Research Centre, Finland. Two mutant potato virus X (PVX) movement protein (MP) genes (m 12K-Sal and m 12K-Kpn) were obtained by inserting specific linkers at the boundary between the N-terminal hydrophobic and putative transmembrane segment, and the central invariant hydrophilic region of the respective 12 kDa, 12K, triple gene block (TGB) protein. Several transgenic potato lines which expressed m 12K-Sal or m 12K-Kpn to different degrees were resistant to infection by PVX, potato aucuba mosaic potexvirus and the carlaviruses potato virus M and S over a wide range of inoculum concentrations (3-300 micrograms/ml). However, they were not resistant to potato virus Y, which lacks a TGB protein. We suggest that the resistance of m 12K-Sal and m 12K-Kpn transgenic potato lines is MP- derived and not RNA-mediated.