Meiosis of anther culture regenerants in asparagus (Asparagus officinalis L.)

Pollen mother cells obtained from regenerated plants of asparagus (Asparagus officinalis L.), in a population composed exclusively of male plants, through the process of anther culture from the hybrid G27 X 22-8, were analyzed during meiosis. It was observed that, during theprocess of anther culture...

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Published inGenetics and molecular biology Vol. 21; no. 1; pp. 93 - 97
Main Authors Galli, Leonardo, Viégas, Judith, Augustin, Eliane, Eckert, Marcia Ines, Silva, João Baptista da
Format Journal Article
LanguageEnglish
Published Sociedade Brasileira de Genética 01.03.1998
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Summary:Pollen mother cells obtained from regenerated plants of asparagus (Asparagus officinalis L.), in a population composed exclusively of male plants, through the process of anther culture from the hybrid G27 X 22-8, were analyzed during meiosis. It was observed that, during theprocess of anther culture by organogenesis, the pollen mother cells of the regenerants had great genomic instability, as evidenced by disturbances in all the meiotic phases of the first and second division. Furthermore, structural chromosomal abnormalities, in addition to aneuploidy and polyploidy, were observed. Foi analisada a meiose em células mãe de pólen de plantas de aspargo (Asparagus officinalis L.) de uma população composta exclusivamente de plantas masculinas, obtidas através do processo de cultura de anteras do híbrido G27 X 22-8. Foi observado que, durante o processo de cultura de anteras, via calogênese, as células mãe de pólen dos regenerantes apresentaram grande instabilidade genômica, evidenciada por irregularidades nas fases de diacinese, assim como de metáfase, anáfase, telófase da primeira e segunda divisão meiótica. Além disto, o processo originou anormalidades cromossômicas estruturais em adição às aneuploidias e poliploidias.
ISSN:1415-4757
1678-4685
1415-4757
1678-4685
DOI:10.1590/S1415-47571998000100016