Mutation of the GKS motif of the RNA-dependent RNA polymerase from potato virus X disables or eliminates virus replication
GF Davenport and DC Baulcombe Sainsbury Laboratory, John Innes Centre, Colney, Norwich, UK. The RNA-dependent RNA polymerase (RdRp) of potato virus X (PVX) contains a glycine-lysine-serine (GKS) motif. This motif is present in the replication enzyme of many RNA viruses and is thought to be required...
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Published in | Journal of general virology Vol. 78; no. 6; pp. 1247 - 1251 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
Soc General Microbiol
01.06.1997
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Subjects | |
Online Access | Get full text |
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Summary: | GF Davenport and DC Baulcombe
Sainsbury Laboratory, John Innes Centre, Colney, Norwich, UK.
The RNA-dependent RNA polymerase (RdRp) of potato virus X (PVX) contains a
glycine-lysine-serine (GKS) motif. This motif is present in the replication
enzyme of many RNA viruses and is thought to be required for nucleoside
triphosphate-binding. Three single amino acid changes, glycine to alanine
(AKS), lysine to asparagine (GNS) and lysine to glutamate (GES) within the
GKS motif of the PVX RdRp were tested for their effect on PVX accumulation.
The GNS and GES mutations rendered the virus unable to accumulate in either
tobacco plants or protoplasts, whereas substitution of glycine with alanine
had only a minor effect on accumulation of PVX. The glycine to alanine
mutation reverted to wild-type after passage on Nicotiana clevelandii
plants. These findings suggest that the GKS motif is required for PVX
replication and that strong selection pressures are active to maintain
necessary sequences of the viral RdRp. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-1317 1465-2099 |
DOI: | 10.1099/0022-1317-78-6-1247 |