A designer enzyme for hydrazone and oxime formation featuring an unnatural catalytic aniline residue

• Published in: Nature chemistry Vol. 10; no. 9; pp. 946 - 952
• Main Authors: Drienovská, Ivana, Mayer, Clemens, Dulson, Christopher, Roelfes, Gerard
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 01.09.2018
• Subjects: Amino acids; Aniline; Catalysis; Catalytic activity; Chains; Design; Embedding; Enzymes; Hydrazones; Hydrophobicity; Lactococcus lactis; Transcription
• ISSN: 1755-4330; 1755-4349
• DOI: 10.1038/s41557-018-0082-z

Creating designer enzymes with the ability to catalyse abiological transformations is a formidable challenge. Efforts toward this goal typically consider only canonical amino acids in the initial design process. However, incorporating unnatural amino acids that feature uniquely reactive side chains could significantly expand the catalytic repertoire of designer enzymes. To explore the potential of such artificial building blocks for enzyme design, here we selected p-aminophenylalanine as a potentially novel catalytic residue. We demonstrate that the catalytic activity of the aniline side chain for hydrazone and oxime formation reactions is increased by embedding p-aminophenylalanine into the hydrophobic pore of the multidrug transcriptional regulator from Lactococcus lactis. Both the recruitment of reactants by the promiscuous binding pocket and a judiciously placed aniline that functions as a catalytic residue contribute to the success of the identified artificial enzyme. We anticipate that our design strategy will prove rewarding to significantly expand the catalytic repertoire of designer enzymes in the future.