Identification of a heat shock locus by whole mount electron microscopy of polytene chromosomes
The suitability of whole mount electron microscopy for the localization of gene activity in polytene chromosomes was tested by heat shock induction in Drosophila hydei. The heat shock response of salivary glands is found out to be detrimental to surface spreading properties of salivary gland materia...
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Published in | Hereditas Vol. 103; no. 1; pp. 39 - 46 |
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Main Author | |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.10.1985
Mendelian Society of Lund Blackwell |
Subjects | |
Online Access | Get full text |
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Summary: | The suitability of whole mount electron microscopy for the localization of gene activity in polytene chromosomes was tested by heat shock induction in Drosophila hydei. The heat shock response of salivary glands is found out to be detrimental to surface spreading properties of salivary gland material. The major heat shock band in division 81 is determined to be 81C2. The heat shock puff is covered by amorphous material probably representing transcription complexes that partly obscure the underlying structures. After 5 min heat shock treatment, spherical particles are observed on the puff site. Successful mapping of this region, which consists of very thin bands including a developmental puff, suggests that whole mount electron microscopy is the method of choice for routine localization of gene activity in polytene chromosome systems. |
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Bibliography: | istex:A896B9822A59093344432114451AAFD9E1BFEA75 ark:/67375/WNG-9W20GMX4-1 ArticleID:HRD239 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0018-0661 1601-5223 |
DOI: | 10.1111/j.1601-5223.1985.tb00481.x |