Mass Spectrometry Assisted Lithography for the Patterning of Cell Adhesion Ligands on Self‐Assembled Monolayers

• Published in: Angewandte Chemie International Edition Vol. 48; no. 19; pp. 3507 - 3511
• Main Authors: Kim, Young‐Kwan, Ryoo, Soo‐Ryoon, Kwack, Sul‐Jin, Min, Dal‐Hee
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY‐VCH Verlag 27.04.2009; Wiley
• Subjects: Cell Adhesion; Cell Adhesion Molecules - chemistry; Chemistry; Chemistry, Multidisciplinary; Ethylene Glycols - chemistry; fluorescence imaging; Gold - chemistry; Immobilized Proteins - chemistry; Ligands; lithography; Maleimides - chemistry; mass spectrometry; monolayers; Oligopeptides - chemistry; Physical Sciences; Science & Technology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Surface Properties; GOLD; ATTACHMENT; monolayers; SEQUENCES; fluorescence imaging; lithography; PEPTIDES; cell adhesion; DIP-PEN NANOLITHOGRAPHY; mass spectrometry; MICROARRAYS; PROTEINS; SOFT LITHOGRAPHY; COCULTURES; SURFACES
• ISSN: 1433-7851; 1521-3773
• DOI: 10.1002/anie.200806098

Pattern of events: A simple and flexible method has been developed for patterning cell adhesion ligands. Locally erasing self‐assembled monolayers with tri(ethyleneglycol) groups on a gold substrate by using a MALDI‐TOF MS nitrogen laser and filling the exposed gold surface with an alkanethiol presenting carboxylic acid groups enables subsequent immobilization of maleimide and a cell adhesion peptide, which can then recognize cells (see scheme). Pattern of events: A simple and flexible method has been developed for patterning cell adhesion ligands. Locally erasing self‐assembled monolayers with tri(ethyleneglycol) groups on a gold substrate by using a MALDI‐TOF MS nitrogen laser and filling the exposed gold surface with an alkanethiol presenting carboxylic acid groups enables subsequent immobilization of maleimide and a cell adhesion peptide, which can then recognize cells (see scheme).