Differential accumulation of Xanthomonas campestris pv. campestris proteins during the interaction with the host plant: Contributions of an in vivo system

• Published in: Proteomics (Weinheim) Vol. 17; no. 12
• Main Authors: Santos, Cristiane, Maximiano, Mariana R., Ribeiro, Daiane G., Oliveira‐Neto, Osmundo B., Murad, André M., Franco, Octávio L., Mehta, Angela
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.06.2017
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Black rot; Black rot disease; Brassica; Brassica - growth & development; Brassica - metabolism; Brassica - microbiology; Cell culture; Gene expression; Host plants; Host-Pathogen Interactions; In vivo methods and tests; Label‐free proteomics; Pathogenicity; Pathogens; Plant Diseases - microbiology; Plant extracts; Plant Leaves - growth & development; Plant Leaves - metabolism; Plant Leaves - microbiology; Plant–pathogen interaction; Proteins; Proteome - metabolism; Two dimensional analysis; Virulence Factors - genetics; Virulence Factors - metabolism; Xanthomonas campestris; Xanthomonas campestris - pathogenicity; Plant-pathogen interaction; Black rot disease; Label-free proteomics
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.201700086

Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot, a highly destructive disease that affects all brassicas. This work aimed to study the interaction Xcc–Brassica oleracea using an in vivo system in an attempt to identify proteins involved in pathogenicity. We used label‐free shotgun 2D‐nanoUPLC/MSE to analyze Xcc proteins in three conditions: in the interaction with susceptible (REK) and resistant (REU) plants and in culture medium (control condition). A model of Xcc–susceptible host interaction is proposed and shows that Xcc increases the abundance of several crucial proteins for infection and cell protection. In this study, we also confirmed the differential expression by qPCR analysis of selected genes. This is the first report showing a large‐scale identification of proteins in an in vivo host plant condition. Considering that most studies involving phytopathogens are in vitro (growth in culture medium or in plant extract), this work contributes with relevant information related to the plant–pathogen interaction in planta.