Soluble 80-kd fragment of cell-CAM 120/80 disrupts cell-cell adhesion

Calcium-dependent cell adhesion molecules (CAMs) mediate intercellular adhesion in epithelial cells and in preimplantation mammalian embryos. One of these molecules, cell-CAM 120/80, is found on cells as a 120-kd membrane glycoprotein and as a soluble 80-kd species in conditioned culture medium [Dam...

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Bibliographic Details
Published inJournal of cellular biochemistry Vol. 34; no. 3; p. 187
Main Authors Wheelock, M J, Buck, C A, Bechtol, K B, Damsky, C H
Format Journal Article
LanguageEnglish
Published United States 01.07.1987
Subjects
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Antigens, Surface - isolation & purification
Cell Adhesion
Cell Adhesion Molecules
Cell Line
Cell Membrane - immunology
Chromatography, Affinity
Humans
Molecular Weight
Solubility
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Summary:Calcium-dependent cell adhesion molecules (CAMs) mediate intercellular adhesion in epithelial cells and in preimplantation mammalian embryos. One of these molecules, cell-CAM 120/80, is found on cells as a 120-kd membrane glycoprotein and as a soluble 80-kd species in conditioned culture medium [Damsky et al: Cell 34:455, 1983]. We have purified to homogeneity the soluble 80-kd fragment of cell-CAM 120/80 by using monoclonal antibody affinity chromatography. We have shown that the purified molecule can disrupt cell-cell adhesion in cultured epithelial cells, thus indicating that it is directly involved in the adhesive process. In addition, we have further characterized both the 120-kd cell-associated molecule and its 80-kd fragment, including N-terminal sequence analysis.
ISSN:0730-2312
DOI:10.1002/jcb.240340305