DNA-loaded porous polyethersulfone particles for environmental applications I. preparation

• Published in: Journal of applied polymer science Vol. 98; no. 4; pp. 1668 - 1673
• Main Authors: Zhao, Changsheng, Sun, Shudong, Yang, Kaiguang, Nomizu, Motoyoshi, Nishi, Norio
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 15.11.2005; Wiley
• Subjects: Applied sciences; DNA; Exact sciences and technology; Forms of application and semi-finished materials; microsphere structure; Miscellaneous; poly(ether sulfone); Polymer industry, paints, wood; porous particles; preparation; Technology of polymers; poly(ether sulfone); Particle size; Microsphere; Liquid solid adsorption; Ethersulfone polymer; Experimental study; Property processing relationship; Porous material; preparation; Polyelectrolyte; microsphere structure; porous particles; DNA; Phase separation; Manufacturing; Porosity; Adsorbent; Nucleic acid; Growth from solution
• ISSN: 0021-8995; 1097-4628
• DOI: 10.1002/app.22335

DNA‐loaded porous poly(ether sulfone) (PES) particles are prepared for environmental applications by means of a liquid–liquid phase separation technique. The particles prepared by this method have very large porosity and specific surface area, which are useful as absorbents. By manipulating the fabrication conditions, we could control the microsphere structure, including the diameter and porosity of the particles, the pore size, and the specific surface area. Increasing the polymer concentration, which causes lower porosity and smaller pores on the outer surface of the microspheres, led to increased stability of the DNA‐loaded particles and high DNA incorporation efficiency. The DNA‐loaded porous PES particles could accumulate and remove a DNA intercalating pollutant, ethidium bromide (EB). Both the DNA amount incorporated in the particles and the microsphere structure have great effect on the EB removal ratio. These results indicate that the DNA‐loaded PES particles have the potential to be used in environmental applications, which will be further discussed in the following paper. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 98: 1668–1673, 2005