In Vivo Dynamics of Human Stem Cell Repopulation in NOD/SCID Mice

• Published in: Annals of the New York Academy of Sciences Vol. 938; no. 1; pp. 184 - 190
• Main Authors: DICK, JOHN E., GUENECHEA, GUILLERMO, GAN, OLGA I., DORRELL, CRAIG
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.06.2001
• Subjects: Animals; Cell Division; Clone Cells; DNA, Viral - analysis; DNA, Viral - genetics; Gene transfer; Genes, Reporter; Genetic Markers; Genetic Vectors - genetics; Graft Survival; Green Fluorescent Proteins; Hematopoiesis; Hematopoietic Stem Cell Transplantation; Humans; Lentivirus; Luminescent Proteins - analysis; Luminescent Proteins - genetics; Mice; Mice, Inbred NOD; Mice, SCID; NOD/SCID mice; Proviruses - genetics; Radiation Chimera; Recombinant Fusion Proteins - analysis; Retroviridae - genetics; Retrovirus; Severe Combined Immunodeficiency - pathology; Severe Combined Immunodeficiency - therapy; Species Specificity; Stem cells; Transfection; Transgenes; Transplantation, Heterologous; Virus Integration - genetics
• ISSN: 0077-8923; 1749-6632
• DOI: 10.1111/j.1749-6632.2001.tb03588.x

: Primitive human hematopoietic cells can be assayed on the basis of their ability to repopulate immune‐deficient NOD/SCID mice and have been termed SCID repopulating cells (SRCs). The in vivo biological fate of individual SRCs can be tracked by following the unique retroviral insertion site in the progency of transduced SRCs. Distinct human SRCs were identified that differ in the proliferative and self‐renewal capacity indicating that the primitive cell compartment is functionally heterogeneous.