Possible Host Adaptation as an Evolution Factor of Cowpea aphid-borne mosaic virus Deduced by Coat Protein Gene Analysis

• Published in: Journal of phytopathology Vol. 160; no. 2; pp. 82 - 87
• Main Authors: Nicolini, Cícero, Rabelo Filho, Francisco A. C., Resende, Renato O., Andrade, Genira. P., Kitajima, Elliot W., Pio-Ribeiro, Gilvan, Nagata, Tatsuya
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.02.2012; Blackwell; Wiley Subscription Services, Inc
• Subjects: Biological and medical sciences; Cassia hoffmannseggi; cowpea; Cowpea aphid-borne mosaic virus; Fundamental and applied biological sciences. Psychology; passion flower; Phytopathology. Animal pests. Plant and forest protection; Plant viruses and viroids; cowpea; Cassia hoffmannseggi; Plant pathogen; Cowpea aphid-borne mosaic virus; Protein; Virus; Plant; Leguminosae; Gene; Dicotyledones; Angiospermae; Cassia; Spermatophyta; Potyvirus; passion flower; Potyviridae; Adaptation
• ISSN: 0931-1785; 1439-0434
• DOI: 10.1111/j.1439-0434.2011.01861.x

Cowpea aphid‐borne mosaic virus (CABMV) causes major diseases in cowpea and passion flower plants in Brazil and also in other countries. CABMV has also been isolated from leguminous species including, Cassia hoffmannseggii, Canavalia rosea, Crotalaria juncea and Arachis hypogaea in Brazil. The virus seems to be adapted to two distinct families, the Passifloraceae and Fabaceae. Aiming to identify CABMV and elucidate a possible host adaptation of this virus species, isolates from cowpea, passion flower and C. hoffmannseggii collected in the states of Pernambuco and Rio Grande do Norte were analysed by sequencing the complete coat protein genes. A phylogenetic tree was constructed based on the obtained sequences and those available in public databases. Major Brazilian isolates from passion flower, independently of the geographical distances among them, were grouped in three different clusters. The possible host adaptation was also observed in fabaceous‐infecting CABMV Brazilian isolates. These host adaptations possibly occurred independently within Brazil, so all these clusters belong to a bigger Brazilian cluster. Nevertheless, African passion flower or cowpea‐infecting isolates formed totally different clusters. These results showed that host adaptation could be one factor for CABMV evolution, although geographical isolation is a stronger factor.