Detection of Maternal DNA in Umbilical Cord Plasma by Fluorescent PCR Amplification of Short Tandem Repeat Sequences

: Recently, maternal DNA was detected in umbilical cord blood using PCR amplification of minisatellite sequences. The presence of maternal DNA was demonstrated in 1% to 100% of umbilical cord blood samples. The objective of this study was to determine the frequency of cord blood contamination by mat...

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Bibliographic Details
Published inAnnals of the New York Academy of Sciences Vol. 945; no. 1; pp. 161 - 163
Main Authors BAUER, MARGIT, ORESCOVIC, IRMGARD, SCHOELL, WOLFGANG M., BIANCHI, DIANA W., PERTL, BARBARA
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.09.2001
Subjects
DNA - blood
Female
Fetal Blood - chemistry
Fluorescence
Fluorescent PCR
Genetic Markers
Humans
Maternal DNA
Mothers
Polymerase Chain Reaction - methods
Pregnancy
Short tandem repeat (STR)
Tandem Repeat Sequences
Umbilical cord plasma
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Summary:: Recently, maternal DNA was detected in umbilical cord blood using PCR amplification of minisatellite sequences. The presence of maternal DNA was demonstrated in 1% to 100% of umbilical cord blood samples. The objective of this study was to determine the frequency of cord blood contamination by maternal genetic material. We used fluorescent PCR amplification of highly polymorphic short tandem repeat (STR) markers to detect maternal DNA in umbilical cord plasma.
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ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.2001.tb03880.x