Cell injury triggers actin polymerization to initiate epithelial restitution

• Published in: Journal of cell science Vol. 131; no. 16
• Main Authors: Aihara, Eitaro, Medina-Candelaria, Neisha M, Hanyu, Hikaru, Matthis, Andrea L, Engevik, Kristen A, Gurniak, Christine B, Witke, Walter, Turner, Jerrold R, Zhang, Tongli, Montrose, Marshall H
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Ltd 15.08.2018
• Subjects: Actins - metabolism; Animals; Cell Movement; Cells, Cultured; Epithelial Cells - physiology; Female; Gastric Mucosa - injuries; Gastric Mucosa - metabolism; Gastric Mucosa - physiology; Male; Mice; Mice, Transgenic; Organoids - cytology; Organoids - injuries; Organoids - physiology; Polymerization; Protein Multimerization - physiology; Re-Epithelialization - physiology; Regeneration - physiology; Stomach - cytology; Stomach - injuries; Epithelial cell; Gastric organoid; Repair; Actin; Cell migration
• ISSN: 0021-9533; 1477-9137
• DOI: 10.1242/jcs.216317

The role of the actin cytoskeleton in the sequence of physiological epithelial repair in the intact epithelium has yet to be elucidated. Here, we explore the role of actin in gastric repair and gastric organoids (gastroids). In response to two-photon-induced cellular damage of either an gastric or gastroid epithelium, actin redistribution specifically occurred in the lateral membranes of cells neighboring the damaged cell. This was followed by their migration inward to close the gap at the basal pole of the dead cell, in parallel with exfoliation of the dead cell into the lumen. The repair and focal increase of actin was significantly blocked by treatment with EDTA or the inhibition of actin polymerization. Treatment with inhibitors of myosin light chain kinase, myosin II, trefoil factor 2 signaling or phospholipase C slowed both the initial actin redistribution and the repair. While Rac1 inhibition facilitated repair, inhibition of RhoA/Rho-associated protein kinase inhibited it. Inhibitors of focal adhesion kinase and Cdc42 had negligible effects. Hence, initial actin polymerization occurs in the lateral membrane, and is primarily important to initiate dead cell exfoliation and cell migration to close the gap.