Flavobacterium proteolyticum sp. nov., isolated from aquaculture water

• Published in: Archives of microbiology Vol. 204; no. 2; p. 146
• Main Authors: Du, Juan, Liu, Yang, Pei, Tao, Li, Anzhang, Deng, Ming-Rong, Zhu, Honghui
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2022; Springer Nature B.V
• Subjects: Aquaculture; Archives & records; Bacterial Typing Techniques; Biochemistry; Biomedical and Life Sciences; Biotechnology; Casein; Cell Biology; chemotaxonomy; China; Chromatography; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Ecology; Fatty acids; Flavobacterium; Flavobacterium - genetics; Genomes; Genomics; Glucose; glycolipids; Hybridization; hydrolysis; Laboratories; Life Sciences; Lipids; Microbial Ecology; Microbiology; New species; nucleic acid hybridization; Nucleotides; Original Paper; phenotype; Phosphatidylethanolamine; phosphatidylethanolamines; Phylogenetics; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sodium chloride; Software; Taxonomy; Water; Water analysis; Water sampling; China; Genotypes and phenotypes; Polyphasic taxonomy; Genomic analysis; Novel species; Flavobacterium proteolyticum
• ISSN: 0302-8933; 1432-072X; 1432-072X
• DOI: 10.1007/s00203-021-02744-2

A novel aerobic, yellow, and rod-shaped bacterial isolate, designated as 1Y8A T , was isolated from aquaculture water sampled in Jiangmen, Guangdong province, P. R. China. Here, the taxonomic position of strain 1Y8A T was conducted based on phenotypic, genomic, and chemotaxonomic characteristics. Strain 1Y8A T was observed to grow at 10–37 °C (optimum 28 °C), at pH 6.0–9.0 (optimum 7.0) and in 0–2% NaCl (optimum 1%, w/v). The 16S rRNA gene-based analysis showed that strain 1Y8A T was closely related to “ Flavobacterium sasangense ” YC6274 T (99.3%), Flavobacterium aquaticum JC164 T (98.4%), Flavobacterium cucumis R2A45-3 T (98.0%), Flavobacterium celericrescens TWA-26 T (98.0%), and Flavobacterium cheniae NJ-26 T (97.2%). The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain 1Y8A T and reference strains above were far below the recognized thresholds of 70% dDDH and 95–96% ANI for species definition, implying that the strain represents a novel genospecies. The phylogenomic analysis indicated that strain 1Y8A T formed an independent branch distinct from known species. The major cellular fatty acids of strain 1Y8A T were iso-C 15:0 , iso-C 15:1 G and C 15:0 ; the polar lipids comprised phosphatidylethanolamine, glycolipid, and two lipids; the respiratory quinone was MK-6. The G + C content of genomic DNA was 32.5%. Based on the genotypic and phenotypic characteristics such as the utilization of D-glucose and casein hydrolysis, strain 1Y8A T is concluded to represent a novel species of the genus Flavobacterium , for which the name Flavobacterium proteolyticum sp. nov. is proposed. The type strain of the species is 1Y8A T (= GDMCC 1.1933 T  = KACC 22081 T ).