Mucosal Protective Effect of PGI2 on Canine Small Bowel Auto-transplantation

• Published in: Transplantation proceedings Vol. 44; no. 4; pp. 1169 - 1170
• Main Authors: Kim, Y.I, Chung, K.-Y, So, B.J, Park, J.J
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier Inc 01.05.2012; Elsevier
• Subjects: Animals; Biological and medical sciences; Cytoprotection; Dogs; Endotoxins - blood; Epoprostenol - administration & dosage; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Ileum - drug effects; Ileum - metabolism; Ileum - pathology; Ileum - transplantation; Infusions, Intra-Arterial; Interleukin-6 - blood; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Intestinal Mucosa - transplantation; Jejunum - drug effects; Jejunum - metabolism; Jejunum - pathology; Jejunum - transplantation; Medical sciences; Mesenteric Arteries; Surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Time Factors; Tissue, organ and graft immunology; Transplantation, Autologous; Fissipedia; Carnivora; Digestive system; Mucosa; Protective factor; Transplantation; Homotransplantation; Small intestine; Medicine; Vertebrata; Mammalia; Treatment; Surgery; Animal; Graft; Dog; Protection
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2012.02.025

Abstract Purpose We designed this experimental study to assess the mucosal protective effects of continuous prostaglandin I2 (PGI2) infusion after canine small bowel autotransplantation. Materials and Methods Six Mongrel dogs were randomly divided two groups: PGI2 (n = 3) and control (n = 3). The small bowel from jejunum to ileum was obtained, including the mesenteric vascular pedicle. After cold flushing ex vivo, the harvested segment was preserved in an icebox for 3 hours. Thereafter we reimplanted the harvested intestinal segment. While completing the anastomosis, PGI2 (50 μg) was slowly infused through the mesenteric artery in the PGI2 group versus the same volume of saline in the control group. At 1, 3 and 6 days after autotransplantation, we obtained blood samples, and at 6 days, small bowel segments. Endotoxin and interleukin 6 (IL-6) levels were measured and all histologic specimens stained with hematoxylin-eosin H-E were reviewed by a pathologist to grade mucosal damage as: mild (1 point), moderate (2 points), or severe (3 points) change. Results Mean basal serum endotoxin levels were similar in both groups the PGI2 groups versus control group were 0.216 ± 0.018 versus 0.223 ± 0.040 EU/mL, respectively. However, on day 3 after the operation, the PGI2 group showed much decreased levels of serum endotoxin compared to control levels: 0.349 ± 0.196 versus 0.842 ± 0.446 EU/mL. The mean concentration of serum IL-6 on day 1 after operation among the PGI2 versus control group were 32.13 ± 7.13 pg/mL versus 36.96 ± 3.65 pg/mL. The histologic scores at 6 days after the operation were PGI2 group versus control group: 1.33 versus 1.66 ( P = NS). Conclusion Continuous infusions of PGI2 through the mesenteric artery after the canine small bowel autotransplantation may protect the small bowel mucosal barrier.