Identification of an anti-inflammatory potential of Eriodictyon angustifolium compounds in human gingival fibroblasts
Polyphenol-rich plant extracts have been shown to possess anti-inflammatory activity against oral pathogen-induced cytokine release in model systems of inflammation. Here, it was hypothesized that a flavanone-rich extract of E. angustifolium exhibits an anti-inflammatory potential against endotoxin-...
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Published in | Food & function Vol. 7; no. 7; pp. 346 - 355 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
13.07.2016
|
Subjects | |
Online Access | Get full text |
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Summary: | Polyphenol-rich plant extracts have been shown to possess anti-inflammatory activity against oral pathogen-induced cytokine release in model systems of inflammation. Here, it was hypothesized that a flavanone-rich extract of
E. angustifolium
exhibits an anti-inflammatory potential against endotoxin-induced inflammatory response in human gingival fibroblasts (HGF-1). HGF-1 cells were stimulated with lipopolysaccharide from
Porphyromonas gingivalis
(
pg
-LPS) to release pro-inflammatory cytokines. Concentrations of interleukins IL-6 and IL-8 and macrophage chemoattractant protein-1 in the incubation media upon stimulation were determined by means of magnetic bead analysis. A crude ethanol/water extract of
E. angustifolium
(EE) was fractionated
via
gel permeation chromatography into a flavanone-rich fraction (FF) and an erionic acid-rich fraction (EF). Individual flavanones and erionic acids as well as EE, EF and FF were tested in the
pg
-LPS-stimulated HGF-1 cells for their anti-inflammatory potential. The
E. angustifolium
extract possessed anti-inflammatory potential in this model system, attenuating the
pg
-LPS-induced release of IL-6 by up to 52.0 ± 15.5%. Of the individual flavanones, eriodictyol and naringenin had the most pronounced effect. However, a mixture of the flavanones did not possess the same effect as the entire flavanoid fraction, indicating that other compounds may contribute to the anti-inflammatory potential of
E. angustifolium
. For the first time, an anti-inflammatory potential of
E. angustifolium
and containing erionic acids has been determined.
Erionic acids, flavanones and plant extracts possess anti-inflammatory activity against oral pathogen-induced cytokine release in HGF-1 cells. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2042-6496 2042-650X |
DOI: | 10.1039/c6fo00482b |