Reconstitution of the response to leukemia inhibitory factor, oncostatin M, and ciliary neurotrophic factor in hepatoma cells

• Published in: The Journal of biological chemistry Vol. 268; no. 12; pp. 8414 - 8417
• Main Authors: BAUMANN, H, ZIEGLER, S. F, MOSLEY, B, MORELLA, K. K, PAJOVIC, S, GEARING, D. P
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 25.04.1993
• Subjects: Acute-Phase Proteins - metabolism; Analysis of the immune response. Humoral and cellular immunity; Animals; Biological and medical sciences; Carcinoma, Hepatocellular; Ciliary Neurotrophic Factor; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Growth Inhibitors - metabolism; Humans; Immunobiology; Interleukin-6; Leukemia Inhibitory Factor; Leukemia Inhibitory Factor Receptor alpha Subunit; Liver - metabolism; Lymphokines - metabolism; Lymphokines, interleukins ( function, expression); Mice; Nerve Tissue Proteins - metabolism; Oncostatin M; Peptides - metabolism; Rats; Receptor, Ciliary Neurotrophic Factor; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; Receptors, Cytokine; Receptors, Immunologic - genetics; Receptors, Immunologic - metabolism; Receptors, OSM-LIF; Regulatory factors and their cellular receptors; Transfection; Tumor Cells, Cultured; Vertebrata; Acute phase protein; Mammalia; Rat; Liver cell carcinoma; Leukemia inhibitor factor; Cytokine; Rodentia; Gene expression
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)52890-8

Ciliary neurotrophic factor (CNTF) has been described as a neuro-active cytokine that shares functional similarities with the leukemia inhibitory factor (LIF). We demonstrate here that, like LIF, CNTF stimulates expression of acute phase plasma proteins in rat H-35 hepatoma cells. Transfection of the LIF receptor into Hep3B hepatoma cells reconstituted LIF and oncostatin M regulation of acute phase plasma protein genes. Co-expression of the LIF receptor and the CNTF receptor, but not expression of either subunit alone, generated CNTF responsiveness in Hep3B cells, suggesting cooperativity of these receptor subunits. Evidence is presented for direct interaction of the LIF receptor with the intracellular signal transduction machinery.