Isolation of Cultured Endothelial Progenitor Cells in vitro from PBMCs and CD133~+ Enriched Cells

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 30; no. 1; pp. 18 - 24
• Main Author: 郑伟红 万亚峰 马小鹏 李兴睿 杨志芳 殷茜 易继林
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.02.2010; Department of General Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
• Subjects: AC133 Antigen; Antigens, CD - metabolism; Cell Separation - methods; Cells, Cultured; Endothelial Cells - cytology; Endothelial Cells - metabolism; Glycoproteins - metabolism; Hematopoietic Stem Cells - cytology; Humans; Leukocytes, Mononuclear - cytology; Medicine; Medicine & Public Health; Peptides - metabolism; Vascular Endothelial Growth Factor A - metabolism; cell culture; endothelial progenitor cells; MACS
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-010-0104-6

Two isolation methods for sorting of endothelial progenitor cells(EPCs):from peripheral blood mononuclear cells(PBMCs)and CD133+ enriched cells were compared,by defining the cell morphology,phenotype,reproductive activities and function in vitro,to provide a reference for clinical application of EPCs.PBMCs from healthy subjects were used either directly for cell culture or for CD133+ sorting.The two groups of cells were cultured in complete medium 199(M199)for 7 to 14 days and the phenotypes of EPCs were an...