Identification and Defensive Characterization of PmCYP720B11v2 from Pinus massoniana

• Published in: International journal of molecular sciences Vol. 23; no. 12; p. 6640
• Main Authors: Liu, Bin, Xie, Yini, Yin, Huanhuan, Zhou, Zhichun, Liu, Qinghua
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14.06.2022; MDPI
• Subjects: Adaptation; Animals; Biosynthesis; China; Cytochrome; Cytochrome P450; Cytochrome P450 monooxygenase; Cytochromes P450; Defense mechanisms; defense response; Diterpenes; Drought; drought stress tolerance; Enzymes; Epidemics; Genes; Inoculation; Localization; Metabolic engineering; Metabolic flux; Metabolism; Metabolites; Microorganisms; Nematodes; Oleoresins; Pest resistance; pine wood nematode; Pinus - genetics; Pinus massoniana; Plant Diseases - genetics; PmCYP720B11v2; Proteins; Terpenes; tissue-specific expression; Trees; Tylenchida - physiology; China; tissue-specific expression; defense response; pine wood nematode; PmCYP720B11v2; Pinus massoniana; drought stress tolerance
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23126640

is a pioneer species for afforestation timber and oleoresin, while epidemics of pinewood nematode (PWN; ) are causing a serious biotic disaster for in China. Importantly, resistant could leak copious oleoresin terpenoids to build particular defense fronts for survival when attacked by PWN. However, the defense mechanisms regulating this process remain unknown. Here, , a cytochrome P450 monooxygenase gene, was first identified and functionally characterized from resistant following PWN inoculation. The tissue-specific expression pattern and localization of at the transcript and protein levels in resistant indicated that its upregulation in the stem supported its involvement in the metabolic processes of diterpene biosynthesis as a positive part of the defense against PWN attack. Furthermore, overexpression of may enhance the growth and development of plants. In addition, activated the metabolic flux of antioxidases and stress-responsive proteins under drought conditions and improved drought stress tolerance. Our results provide new insights into the favorable role of in diterpene defense mechanisms in response to PWN attack in resistant and provide a novel metabolic engineering scenario to reform the stress tolerance potential of tobacco.