Production and characterization of the lipopeptide with anti-adhesion for oral biofilm on the surface of titanium for dental implants

• Published in: Archives of microbiology Vol. 206; no. 8; p. 354
• Main Authors: Carvalho, Fernanda Souza, Tarabal, Vinícius Souza, Livio, Diego Fernandes, Cruz, Luísa F., Monteiro, Ana P.F., Parreira, Adriano Guimarães, Guimarães, Pedro P.G., Scheuerman, Karina, Chagas, Rafael Cesar Russo, da Silva, José Antônio, Gonçalves, Daniel Bonoto, Granjeiro, José Mauro, Sinisterra, Ruben Dario, Segura, Maria E.C., Granjeiro, Paulo Afonso
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2024; Springer Nature B.V
• Subjects: Actinobacillus actinomycetemcomitans; Adhesion; Aggregatibacter actinomycetemcomitans; Aggregatibacter actinomycetemcomitans - drug effects; Anti-Bacterial Agents - pharmacology; Antimicrobial activity; Antimicrobial agents; antimicrobial properties; Archives & records; Bacillus subtilis; Bacillus subtilis - drug effects; Bacteria; bacterial adhesion; Bacterial Adhesion - drug effects; Biochemistry; biofilm; Biofilms; Biofilms - drug effects; Biofilms - growth & development; Biomedical and Life Sciences; Biosurfactants; Biotechnology; Cell Biology; Cell Survival - drug effects; Cell viability; Cytotoxicity; Dental implants; Dental Implants - microbiology; Dental prosthetics; Ecology; fibroblasts; Fibroblasts - drug effects; Fusobacterium nucleatum; Fusobacterium nucleatum - drug effects; growth retardation; Gum disease; Humans; Hydrophobicity; Life Sciences; lipopeptides; Lipopeptides - pharmacology; Micelles; Microbial Ecology; Microbial Sensitivity Tests; Microbiology; Microorganisms; Minimum inhibitory concentration; Original Paper; osteoblasts; Osteoblasts - drug effects; Peptides; Porphyromonas gingivalis; Porphyromonas gingivalis - drug effects; Porphyromonas gingivalis - growth & development; Porphyromonas gingivalis - physiology; Proteus mirabilis; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - physiology; Streptococcus infections; Streptococcus sanguinis; Surface Properties; Surface-Active Agents - pharmacology; Surfactants; Surgical implants; Titanium; Titanium - chemistry; Titanium - pharmacology; Titanium alloys; Toxicity; Titanium; Lipopeptide; Oral biofilm; Anti-adhesion
• ISSN: 0302-8933; 1432-072X; 1432-072X
• DOI: 10.1007/s00203-024-04078-1

Titanium implants are subject to bacterial adhesion and peri-implantitis induction, and biosurfactants bring a new alternative to the fight against infections. This work aimed to produce and characterize the biosurfactant from Bacillus subtilis ATCC 19,659, its anti-adhesion and antimicrobial activity, and cell viability. Anti-adhesion studies were carried out against Streptococcus sanguinis , Staphylococcus aureus , Fusobacterium nucleatum , Aggregatibacter actinomycetemcomitans , Porphyromonas gingivalis , and Proteus mirabilis as the minimum inhibitory concentration and the minimum bactericidal concentration. Cell viability was measured against osteoblast and fibroblast cells. The biosurfactant was classified as lipopeptide, with critical micelle concentration at 40 µg mL − 1 , and made the titanium surface less hydrophobic. The anti-adhesion effect was observed for Staphylococcus aureus and Streptococcus sanguinis with 54% growth inhibition and presented a minimum inhibitory concentration of 15.7 µg mL − 1 for Streptococcus sanguinis and Aggregatibacter actinomycetemcomitans . The lipopeptide had no cytotoxic effect and demonstrated high potential application against bacterial biofilms. Graphical Abstract