Dissociation, culture and morphologic changes of interstitial cells of Cajal in vitro
AIM: To study the method of dissociation, culture and investigate its morphologic changes in vitro of interstitial cells of Cajal (ICC).METHODS: Enzymatic digestion and Ficoll density centrifugation were used to dissociate ICC from the ileal segment of mice. Factors including contamination, Ca2+, Mg...
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Published in | World journal of gastroenterology : WJG Vol. 11; no. 18; pp. 2838 - 2840 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Department of General Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China
14.05.2005
Baishideng Publishing Group Inc |
Subjects | |
Online Access | Get full text |
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Summary: | AIM: To study the method of dissociation, culture and investigate its morphologic changes in vitro of interstitial cells of Cajal (ICC).METHODS: Enzymatic digestion and Ficoll density centrifugation were used to dissociate ICC from the ileal segment of mice. Factors including contamination, Ca2+, Mg2+ and collagenase, and stem cell factor, etc, were investigated.ACK2, the antibody of c-kit, was used to identify the cultured ICC. Both light microscope and fluorescence microsope were used to observe the changes of ICC in vitro.RESULTS: The method for dissociation and culture of ICC in vitro was successfully established. After 24 h, cultured ICC exhibited a few axis-cylinders, and longer axis-cylinders were observed to form synapse of each other after 3 d.More widespread connections formed within 7 d in vitro.The changes of its morphologic character were obvious within 7 d; however, there were no obvious morphologic changes after 30 d.CONCLUSION: Many factors can influence the dissociation and culture of ICC. |
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Bibliography: | 14-1219/R R329.2 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Correspondence to: Dr. Wei-Dong Tong, Department of General Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China. lichunxue1974@hotmail.com Telephone: +86-23-68757248 Fax: +86-23-68757248 Author contributions: All authors contributed equally to the work. |
ISSN: | 1007-9327 2219-2840 |
DOI: | 10.3748/wjg.v11.i18.2838 |