In vivo tracing of superparamagnetic iron oxide-labeled bone marrow mesenchymal stem cells transplanted for traumatic brain injury by susceptibility weighted imaging in a rat model

• Published in: Chinese journal of traumatology Vol. 13; no. 3; pp. 173 - 177
• Main Author: 程敬亮 杨运俊 李华丽 王娟 王美豪 张勇
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.06.2010; Department of Radiology, First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052, China
• Subjects: Animals; Bone Marrow Cells - cytology; Brain injuries; Brain Injuries - pathology; Brain Injuries - surgery; Cells, Cultured; Dextrans; Echo-planar imaging; Ferrosoferric Oxide; Magnetic Resonance Imaging - methods; Magnetite Nanoparticles; Male; Mesenchymal Stem Cell Transplantation; Mesenchymal stem cells; Rats; Rats, Sprague-Dawley; 创伤性脑损伤; 大鼠模型; 干细胞移植; 超顺磁性氧化铁; 骨髓基质干细胞; Echo-planar imaging; Brain injuries; Mesenchymal stem cells
• ISSN: 1008-1275
• DOI: 10.3760/cma.j.issn.1008-1275.2010.03.008

Objective：To label rat bone marrow mesenchymal stem cells （BMSCs） with superparamagnetic iron oxide （SPIO） in vitro, and to monitor the survival and location of these labeled BMSCs in a rat model of traumatic brain injury （TBI） by susceptibility weighted imaging （SWI）sequence.Methods：BMSCs were cultured in vitro and then labeled with SPIO. Totally 24 male Sprague Dawley （SD） rats weighing 200-250 g were randomly divided into 4 groups： Groups A-D （n=6 for each group）. Moderate TBI models of all the rats were developed in the left hemisphere following Feeney＇s method. Group A was the experimental group and stereotaxic transplantation of BMSCs labeled with SPIO into the region nearby the contusion was conducted in this group 24 hours after TBI modeling. The other three groups were control groups with transplantation of SPIO, unlabeled BMSCs and injection of nutrient solution respectively conducted in Groups B, C and D at the same time. Monitoring of these SPIO-labeled BMSCs by SWI was performed one day,one week and three weeks after implantation.Results： Numerous BMSCs were successfully labeled with SPIO. They were positive for Prussian blue staining and intracytoplasm positive blue stained particles were found under a microscope （×200）. Scattered little iron particles were observed in the vesicles by electron microscopy （×5000）. MRI of the transplantation sites of the left hemisphere demonstrated a low signal intensity on magnitude images,phase images and SWI images for all the test rats in Group A, and the lesion in the left parietal cortex demonstrated a semicircular low intensity on SWI images, which clearly showed the distribution and migration of BMSCs in the first and third weeks. For Group B, a low signal intensity by MRI was only observed on the first day but undetected during the following examination. No signals were observed in Groups C and D at any time points.Conclusion：SWI sequence in vivo can consecutively and noninvasively trace and demonstrate the status and distribution of BMSCs labeled with SPIO in the brain of TBI model rats.