Inhibition of hepatitis B virus production by Boehmeria nivea root extract in HepG2 2.2.15 cells

• Published in: World journal of gastroenterology : WJG Vol. 12; no. 35; pp. 5721 - 5725
• Main Authors: Huang, Kai-Ling, Lai, Yiu-Kay, Lin, Chih-Chien, Chang, Jia-Ming
• Format: Journal Article
• Language: English
• Published: United States Department of Life Sciences and Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan 30013, China%Department of Life Sciences and Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan 30013, China 21.09.2006; Department of Bioresources, Da-Yeh University, Changhua, Taiwan 515, China%Division of Research and Development, Development Center for Biotechnology, Xizhi City, Taipei County, Taiwan 221, China; Baishideng Publishing Group Co., Limited
• Subjects: Antiviral Agents - pharmacology; Boehmeria - chemistry; Carcinoma, Hepatocellular - drug therapy; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Carcinoma, Hepatocellular - virology; Cell Line, Tumor; Cytotoxins - pharmacology; DNA Replication - drug effects; DNA, Viral - genetics; DNA, Viral - metabolism; Gene Expression Regulation, Neoplastic - drug effects; Gene Expression Regulation, Viral - drug effects; Hepatitis B e Antigens - genetics; Hepatitis B e Antigens - metabolism; Hepatitis B Surface Antigens - genetics; Hepatitis B Surface Antigens - metabolism; Hepatitis B virus - drug effects; Hepatitis B virus - genetics; Hepatitis B virus - immunology; Hepatitis B virus - metabolism; Humans; Liver Neoplasms - drug therapy; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Liver Neoplasms - virology; Plant Extracts - analysis; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Plant Roots - chemistry; Rapid Communication; RNA, Viral - genetics; RNA, Viral - metabolism; Transcription, Genetic - drug effects; 乙型肝炎; 病毒感染; 肝炎病毒; Medicinal herb; Anti-hepatitis B virus; Antiviral agent; Hepatitis B virus; Boehmeria nivea; HepG2 2.2.15
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v12.i35.5721

AIM： To explore the anti-hepatitis B virus （HBV） effects of Boehmeria nivea （B. nivea） root extract （BNE） by using the HepG2 2.2.15 cell model system. METHODS： Hepatitis B surface antigen （HBsAg）, hepatitis B virus e antigen （HBeAg）, and HBV DNA were measured by using ELISA and real-time PCR, respectively. Viral DNA replication and RNA expression were determined by using Southern and Northern blot, respectively. RESULTS： In HepG2 2.2.15 cells, HBeAg （60%, P 〈 0.01） and particle-associated HBV DNA （〉 99%, P 〈 0.01） secretion into supernatant were significantly inhibited by BNE at a dose of 100 mg/L, whereas the HBsAg was not inhibited. With different doses of BNE, the reduced HBeAg was correlated with the inhibition of HBV DNA. The anti-HBV effect of BNE was not caused by its cytotoxicity to cells or inhibition of viral DNA replication and RNA expression. CONCLUSION： BNE could effectively reduce the HBV production and its anti-HBV machinery might differ from the nucleoside analogues.