Reprogramming of fetal cells by avian EE for generation of pluripotent stem cell like cells in caprine

• Published in: Research in veterinary science Vol. 95; no. 2; pp. 638 - 643
• Main Authors: Bharadwaj, Parul, Kumar, Kuldeep, Singh, Renu, Puri, Gopal, Yasotha, T., Kumar, Manish, Bhure, Sanjeev, Das, B.C., Sarkar, M., Bag, Sadhan
• Format: Journal Article
• Language: English
• Published: England Elsevier India Pvt Ltd 01.10.2013; Elsevier Limited
• Subjects: Animals; birds; Caprine; Cell Culture Techniques; Cell Differentiation; Cell Extracts - chemistry; Cell Extracts - pharmacology; Chickens; Egg Yolk - chemistry; Extract Egg; Fetal bovine serum; Fetal cells; Gene expression; genes; Genomes; Goats; immunocytochemistry; Pluripotent Stem Cells - cytology; Pluripotent Stem Cells - physiology; Reprogramming; Stem cells; Transplants & implants; Veterinary medicine; Extract Egg; Reprogramming; Fetal cells; Fetal bovine serum; Caprine

The present work was carried out to study the ability of avian “Extract Egg” (EE) for reprogramming caprine fetal cells. The isolated caprine fetal cells were cultured in stem cell media supplemented with different percentages of either EE or FBS. The results indicated that the supplementation of 2–...