Reprogramming of fetal cells by avian EE for generation of pluripotent stem cell like cells in caprine

• Published in: Research in veterinary science Vol. 95; no. 2; pp. 638 - 643
• Main Authors: Bharadwaj, Parul, Kumar, Kuldeep, Singh, Renu, Puri, Gopal, Yasotha, T., Kumar, Manish, Bhure, Sanjeev, Das, B.C., Sarkar, M., Bag, Sadhan
• Format: Journal Article
• Language: English
• Published: England Elsevier India Pvt Ltd 01.10.2013; Elsevier Limited
• Subjects: Animals; birds; Caprine; Cell Culture Techniques; Cell Differentiation; Cell Extracts - chemistry; Cell Extracts - pharmacology; Chickens; Egg Yolk - chemistry; Extract Egg; Fetal bovine serum; Fetal cells; Gene expression; genes; Genomes; Goats; immunocytochemistry; Pluripotent Stem Cells - cytology; Pluripotent Stem Cells - physiology; Reprogramming; Stem cells; Transplants & implants; Veterinary medicine; Extract Egg; Reprogramming; Fetal cells; Fetal bovine serum; Caprine
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2013.06.014

The present work was carried out to study the ability of avian “Extract Egg” (EE) for reprogramming caprine fetal cells. The isolated caprine fetal cells were cultured in stem cell media supplemented with different percentages of either EE or FBS. The results indicated that the supplementation of 2–4% EE formed lesser but larger size stem cell like cell colonies as compared to 6% or 10% EE. The expression of pluripotent genes were comparatively higher in colonies developed in 2% or 4% as compared to 6% or 10% EE. Further, immunocytochemistry revealed that the colonies developed in all percentage of EE expressed pluripotent markers like Oct4, Nanog, TRA-1-60 and TRA-1-81. Our findings indicated that avian EE has the potentiality to reprogram caprine fetal cells into embryonic state which may help in generation of pluripotent stem cells without using viral vector.