Quantification of amygdalin, prunasin, total cyanide and free cyanide in powdered loquat seeds

Amygdalin, prunasin, total cyanide and free cyanide concentrations in 12 powdered loquat seeds were investigated. Loquat (Eriobotrya japonica) is a species of flowering plant in the family Rosaceae, and its fruit is quite popular in Japan. Amygdalin and prunasin were measured using LC-MS/MS. Total c...

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Published inFood additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Vol. 37; no. 9; pp. 1503 - 1509
Main Authors Tanaka, Tomoya, Kimura, Keisuke, Kan, Kimiko, Katori, Yoshiko, Michishita, Kumi, Nakano, Hisako, Sasamoto, Takeo
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis 01.09.2020
Taylor & Francis Ltd
Subjects
amygdalin
Colorimetry
Cyanides
Distillation
Flowering
free cyanide
LC-MS/MS
Loquat
prunasin
Pyridines
Seeds
Steam distillation
total cyanide
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Summary:Amygdalin, prunasin, total cyanide and free cyanide concentrations in 12 powdered loquat seeds were investigated. Loquat (Eriobotrya japonica) is a species of flowering plant in the family Rosaceae, and its fruit is quite popular in Japan. Amygdalin and prunasin were measured using LC-MS/MS. Total cyanide was measured by enzymatic treatment, steam distillation and colorimetric quantification using the pyridine-pyrazolone method. Free cyanide was measured without enzymatic treatment. The mean concentrations of amygdalin, prunasin, total cyanide and free cyanide in powdered loquat seeds were 5900, 760, 410 and 44 mg/kg, respectively. The range of each quantitative value was extensive. Seven out of twelve samples were at risk for exceeding the acute reference dose (ARfD) of cyanide.
Bibliography:ObjectType-Article-1
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ISSN:1944-0049
1944-0057
DOI:10.1080/19440049.2020.1778186