Isomelezitose formation by glucansucrases

Several glucansucrases were surveyed for their ability to produce isomelezitose, a trisaccharide with the structure α-D-glucopyranosyl (1 → 6) β-D-fructofuranosyl (2 ↔ 1) α-D-glucopyranoside. Nearly all strains tested, with one exception, produced at least trace levels of isomelezitose. Yields were...

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Published inCarbohydrate research Vol. 439; pp. 57 - 60
Main Authors Côté, Gregory L., Skory, Christopher D.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Ltd 01.02.2017
Subjects
Acceptor reaction
Bacterial Proteins - chemistry
Bacterial Proteins - isolation & purification
Bacterial Proteins - metabolism
Biocatalysis
Carbohydrate Sequence
Glucansucrase
Glucosyltransferase
Glycosyltransferases - chemistry
Glycosyltransferases - isolation & purification
Glycosyltransferases - metabolism
Isomelezitose
Isomerism
Lactobacillus - chemistry
Lactobacillus - enzymology
Leuconostoc - chemistry
Leuconostoc - enzymology
Leuconostoc mesenteroides - chemistry
Leuconostoc mesenteroides - enzymology
Magnetic Resonance Spectroscopy
Oligosaccharide
Substrate Specificity
Sucrose - chemistry
Trisaccharides - biosynthesis
Trisaccharides - chemistry
Isomelezitose
Glucansucrase
Acceptor reaction
Oligosaccharide
Glucosyltransferase
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Summary:Several glucansucrases were surveyed for their ability to produce isomelezitose, a trisaccharide with the structure α-D-glucopyranosyl (1 → 6) β-D-fructofuranosyl (2 ↔ 1) α-D-glucopyranoside. Nearly all strains tested, with one exception, produced at least trace levels of isomelezitose. Yields were low but significant, ranging from less than 1% to approximately 5% based on sucrose. This trisaccharide may arise in either of two ways: glucopyranosyl transfer to the 6Fru-OH position of sucrose, or to the anomeric OH position of isomaltulose. This study indicates that isomelezitose formation may be a general phenomenon of many glucansucrase reactions. [Display omitted] •Isomelezitose is formed at detectable levels in 11 of 12 randomly selected glucansucrases.•Isomelezitose yield varies, depending on the enzyme source.•Leuconostoc mesenteroides NRRL B-1297 is the best isomelezitose producer found.•Higher sucrose concentrations enhance isomelezitose yields.
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ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2017.01.004