Inflammation-associated pulmonary microbiome and metabolome changes in broilers exposed to particulate matter in broiler houses

• Published in: Journal of hazardous materials Vol. 421; p. 126710
• Main Authors: Shen, Dan, Guo, Zhendong, Huang, Kai, Dai, Pengyuan, Jin, Xiaoming, Li, Yansen, Li, Chunmei
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 05.01.2022
• Subjects: Inflammatory response; Lung injury; Metabolomics; Microbiome; PM exposure; Metabolomics; Inflammatory response; PM exposure; Lung injury; Microbiome
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2021.126710

The particulate matter (PM) in livestock houses, one of the primary sources of atmospheric PM, is not only detrimental to the respiratory health of animals and farmworkers but also poses a threat to the public environment and public health and warrants increased attention. In this study, we investigated the variation in the pulmonary microbiome and metabolome in broiler chickens exposed to PM collected from a broiler house. We examined the pulmonary microbiome and metabolome in broilers, observing that PM induced a visible change in α and β diversity. A total of 66 differential genera, including unclassified_f_Ruminococcaceae and Campylobacter, were associated with pulmonary inflammation. Untargeted metabolomics was utilised to identify 63 differential metabolites induced by PM and correlated with differential bacteria. We observed that PM resulted in injury of the broiler lung and disruption of the microbial community, as well as causing changes in the observed metabolites. These results imply that perturbations to the microbiome and metabolome may play pivotal roles in the mechanism underlying PM-induced broiler lung damage. [Display omitted] •The whole body PM exposure draws near the actual exposure status of broilers.•Microbiome was used to explore the PM-induced lung microbial disorder in broilers.•Metabolome revealed that PM exposure induced lung metabolic disorder in broilers.•PM-induced lung inflammation, microbial and metabolic disorder are closely related.