Histogenesis and reserve dynamics during the maintenance of dormancy and germination in seeds of the basal palm Mauritiella armata

• Published in: Protoplasma Vol. 261; no. 3; pp. 425 - 445
• Main Authors: Fonseca, Patrícia Pereira, Mazzottini-dos-Santos, Hellen Cássia, de Azevedo, Islaine Franciely Pinheiro, Nunes, Yule Roberta Ferreira, Ribeiro, Leonardo Monteiro
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.05.2024; Springer Nature B.V
• Subjects: Amazonia; Arecaceae; Biomedical and Life Sciences; Carbohydrates; Cell Biology; Cell differentiation; cell growth; cerrado; Dormancy; ecosystems; Electron microscopy; Embryos; Endosperm; Germination; histochemistry; Histogenesis; Life Sciences; lipids; Mauritiella armata; morphogenesis; Operculum; Original Article; Pectin; Plant Sciences; Seeds; species; Water content; Zoology; Cerrado Biome; Amazonia; Embryo anatomy; Palm embryo; Seedling development; Histochemistry; Reserve mobilization
• ISSN: 0033-183X; 1615-6102; 1615-6102
• DOI: 10.1007/s00709-023-01905-y

The germination and post-seminal development of Arecaceae are notably complex due to the microscopic dimensions of the embryonic axis, the occurrence of dormancy, and the diversity of reserve compounds. In-depth information on this subject is still limited, especially in terms of the basal sub-family Calamoideae. Mauritiella armata is widely distributed in the Amazon region and is considered a key species in flooded ecosystems ( veredas ) in the Cerrado biome. We sought to describe histogenesis and reserve compound dynamics during the germination of M. armata , as well as the changes in incubated seeds over time. Seeds with their operculum removed (the structure that limits embryonic growth) were evaluated during germination using standard methods of histology, histochemistry, and electron microscopy. Evaluations were also performed on intact seeds incubated for 180 days. The embryos show characteristics associated with recalcitrant seeds of Arecaceae: a high water content (>80%), differentiated vessel elements, and reduced lipid reserves. Both the embryo and endosperm store abundant reserves of proteins, neutral carbohydrates, and pectins. The completion of germination involves cell divisions and expansions in specific regions of the embryo, in addition to the mobilization of embryonic and endospermic reserves through symplastic and apoplastic flows. Intact seeds show dormancy (not germinating for 180 days), but exhibit continuous development associated with cell growth, differentiation, and reserve mobilization. The anatomical and histochemical characters of M. armata seeds indicate an association between recalcitrance and dormancy related to the species’ adaptation to flooded environments.