Norovirus in feces and nasopharyngeal swab of children with and without acute gastroenteritis symptoms: First report of GI.5 in Brazil and GI.3 in nasopharyngeal swab

• Published in: Journal of clinical virology Vol. 87; pp. 60 - 66
• Main Authors: Dábilla, Nathânia, Nunes Vieira Almeida, Tâmera, Carvalho Rebouças Oliveira, Anniely, Kipnis, André, Neres Silva, Thairiny, Souza Fiaccadori, Fabíola, Teixeira de Sousa, Teresinha, de Paula Cardoso, Divina das Dôres, Souza, Menira
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2017
• Subjects: Allergy and Immunology; Asymptomatic NoV infection; Brazil - epidemiology; Caliciviridae Infections - virology; Child; Child, Preschool; Cross-Sectional Studies; Feces - virology; Female; Genotype; Genotypes; Hospitalized children; Humans; Infant; Infant, Newborn; Infectious Disease; Male; Nasopharynx - virology; Norovirus; Norovirus - classification; Norovirus - genetics; Norovirus - isolation & purification; NoV in nasopharyngeal swab; Prevalence; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral - analysis; RNA, Viral - genetics; RT-qPCR; Sequence Analysis, DNA; Viral Load; Virus Shedding; Norovirus; NoV in nasopharyngeal swab; Hospitalized children; Viral load; Asymptomatic NoV infection; RT-qPCR; Genotypes
• ISSN: 1386-6532; 1873-5967
• DOI: 10.1016/j.jcv.2016.12.009

Highlights • This is the first investigation of norovirus in fecal and nasopharyngeal swab samples obtained from the same children. • We report positivity rates in nasopharyngeal swabs of 11.4% from symptomatic and in 6.5% from asymptomatic children. • A considerable genomic variability was found and a higher frequency of GII NoV was detected in fecal samples. • This is the first report of GI.3 in nasopharyngeal swab samples and also the first report of GI.5 NoV in Brazil. • Findings amplify the need to investigate NoV in respiratory tract of children with and without gastroenteritis.