Cluster analysis of bovine respiratory disease (BRD)-associated pathogens shows the existence of two epidemiological patterns in BRD outbreaks

• Published in: Veterinary microbiology Vol. 280; p. 109701
• Main Authors: Calderón Bernal, Johan Manuel, Fernández, Ana, Arnal, José Luis, Baselga, Cristina, Benito Zuñiga, Alfredo, Fernández-Garyzábal, José Francisco, Vela Alonso, Ana Isabel, Cid, Dolores
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2023
• Subjects: Animals; Bacteria; Bovine respiratory disease; Calves; Cattle; Cattle Diseases - epidemiology; Cluster Analysis; Diarrhea Viruses, Bovine Viral; Disease Outbreaks - veterinary; Hierarchical cluster analysis; Mannheimia haemolytica; Pasteurella multocida - genetics; Respiratory Tract Diseases - veterinary; Virus; Virus; Calves; Bacteria; Hierarchical cluster analysis; Bovine respiratory disease
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2023.109701

A hierarchical cluster analysis was used to classify outbreaks of bovine respiratory disease (BRD; n = 156) in natural groups according to the detection of nine pathogens (parainfluenza 3 virus (PI-3), bovine respiratory syncytial virus (BRSV), bovine coronavirus (BCV), bovine viral diarrhea virus (BVDV), and bovine herpesvirus 1 (BHV-1), Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, and Mycoplasma bovis. Pathogens were detected by individual q-PCRs. Two clusters were identified. Cluster 1 was characterized by a relatively high frequency (40–72%) of four BRD-associated viruses, supporting their primary involvement in BRD. Cluster 2 was characterized by frequencies of PI-3, BRSV, or BVDV below 10% each. P. multocida and M. haemolytica were detected with high frequencies in both clusters (P > 0.05), while M. bovis and H. somni showed a significantly higher frequency in cluster 1and 2, respectively. Outbreaks in cluster 1 were associated with preweaning calves younger than 5 months (OR 2.2; 95% CI 1.1–4.5) and with cold months, whereas cluster 2 was associated with fattening calves older than 5 months after arrival to feedlots and without any seasonality. Thus, in addition to the classic epidemiological BRD pattern characterized by the primary involvement of viruses occurring preferably during winter and affecting young calves, there is a second pattern in which viruses would be less relevant, affecting mainly calves older than 5 months at any time of the year. This study allows a better understanding of the BRD epidemiology, which can be useful when implementing management and prophylaxis measures for a better control of this disease. •Hierarchical cluster analysis identified 2 epidemiological patterns in BRD outbreaks.•Pattern 1: high frequency of viruses affecting preferably young calves during winter.•Pattern 2: virus frequencies below 10% indicating a less relevant role of the virus.•Pattern 2 affected mainly calves older than 5 months at any time of the year.