Structural properties and gene-silencing activity of chemically modified DNA-RNA hybrids with parallel orientation

Abstract We report, herein, a new class of RNAi trigger molecules based on the unconventional parallel hybridization of two oligonucleotide chains. We have prepared and studied several parallel stranded (ps) duplexes, in which the parallel orientation is achieved through incorporation of isoguanine...

Full description

Saved in:
Bibliographic Details
Published inNucleic acids research Vol. 46; no. 4; pp. 1614 - 1623
Main Authors Habibian, Maryam, Yahyaee-Anzahaee, Maryam, Lucic, Matije, Moroz, Elena, Martín-Pintado, Nerea, Di Giovanni, Logan Dante, Leroux, Jean-Christophe, Hall, Jonathan, González, Carlos, Damha, Masad J
Format Journal Article
LanguageEnglish
Published England Oxford University Press 28.02.2018
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:Abstract We report, herein, a new class of RNAi trigger molecules based on the unconventional parallel hybridization of two oligonucleotide chains. We have prepared and studied several parallel stranded (ps) duplexes, in which the parallel orientation is achieved through incorporation of isoguanine and isocytosine to form reverse Watson-Crick base pairs in ps-DNA:DNA, ps-DNA:RNA, ps-(DNA-2′F-ANA):RNA, and ps-DNA:2′F-RNA duplexes. The formation of these duplexes was confirmed by UV melting experiments, FRET and CD studies. In addition, NMR structural studies were conducted on a ps-DNA:RNA hybrid for the first time. Finally, we provide evidence for the unprecedented finding that ps-DNA:RNA and ps-DNA:2′F-RNA hybrids can engage the RNAi pathway to silence gene expression in vitro.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
These authors contributed equally to this work as first authors.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gky024