Further studies on the bioconversion of penicillin G into deacetoxycephalosporin G by resting cells of Streptomyces clavuligerus NP-1

• Published in: Applied biochemistry and biotechnology Vol. 81; no. 3; pp. 145 - 152
• Main Authors: BAEZ-VASQUEZ, M. A, ADRIO, J. L, PIRET, J. M, DEMAIN, A. L
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.09.1999; Springer Nature B.V
• Subjects: a-ketoglutaric acid; ascorbic acid; Bacteria; Biochemistry; Bioconversions. Hemisynthesis; Biological and medical sciences; Biomass; Biotechnology; Biotransformation; Cephalosporins - biosynthesis; Conversion; deacetoxycephalosporin C synthase; deacetoxycephalosporin G; Fundamental and applied biological sciences. Psychology; iron; Methods. Procedures. Technologies; Penicillin; penicillin G; Penicillin G - metabolism; Streptomyces - enzymology; Streptomyces - metabolism; Streptomyces clavuligerus; Studies; Actinomycetales; Antibiotic; Cephalosporin derivatives; Streptomycetaceae; Biotransformation; Bacteria; Actinomycetes; Benzylpenicillin; Streptomyces clavuligerus; Antimicrobial agent
• ISSN: 0273-2289; 1559-0291; 0273-2289
• DOI: 10.1385/ABAB:81:3:145

Resting cells of Streptomyces clavuligerus NP-1, which possess deacetoxycephalosporin C synthase activity, have been shown previously to perform oxidative ring expansion of penicillin G in the presence of iron, ascorbic acid, and alpha-ketoglutaric acid to form deacetoxycephalosporin G. Further studies on this bioconversion indicated that use of MOPS or HEPES buffer at pH 6.5 more than doubled the extent of the reaction observed with the previously used Tris-HCl at pH 7.4. Levels of bioconversion as high as 16.5% were achieved at low penicillin G concentrations. Previously, conversion yields were < 1%.