Co-culture of Mesenchymal Stem Cells with Umbilical Vein Endothelial Cells under Hypoxic Condition

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 32; no. 2; pp. 173 - 180
• Main Author: 张波 杨述华 张宇坤 孙志博 许伟华 叶树楠
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.04.2012; Department of Orthopedics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China
• Subjects: Adult; Cell Communication - physiology; Cell Hypoxia - physiology; Cell Movement - physiology; Cell Survival - physiology; cells; Cells, Cultured; co-culture; Coculture Techniques; Cytokines - metabolism; endothelial; Endothelial Cells - cytology; Endothelial Cells - physiology; Female; femoral; head; Humans; hypoxia; Male; Medicine; Medicine & Public Health; mesenchymal; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - physiology; necrosis; Neovascularization, Physiologic - physiology; stem; umbilical; Umbilical Veins - cytology; Umbilical Veins - physiology; vein; umbilical vein endothelial cells; hypoxia; femoral head necrosis; co-culture; mesenchymal stem cells
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-012-0031-9

By co-culturing humm mesenchymal stem cells (hMSCs) and human umbilical rein en-dothelial cells (HUVECs) under hypoxia and creating a microenvironment similar to that of trans-planted hMSCs for the treatment of avascular ni ANFH, the effect of hMSCs on survival, apoptosis, migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) under the hypoxic condition were investigated in vitro. hMSCs and HUVECs were cultured and identified in vitro. Three kinds of conditioned media, CdM-CdMNOR, CdM-CdMHYP and HUVEC-CdMHYP were pre-pared. HUVECs were cultured with these conditioned media under hypoxia. The survival rate, apop-tosis rate, migration and angiogenesis of HUVECs were respectively detected by CCK-8, flow cy-tometry, Transwell and tube formation assay. The content of SDF-1α, VEGF and IL-6 in CdM was determined by ELISA. Our results showed that hMSCs and HUVECs were cultured and identified successfully. Compared with MSC-CdMNOR and HUVEC-CdMHYP groups, the survival rate, migra-tion and angiogenesis of HUVECs in MSC-CdMHYP group were significantly increased while the apoptosis rate was declined (P0.05). Moreover, the expression of SDF-1α, VEGF and IL-6 in MSC-CdMHYP group was up-regulated. Under hypoxia, the apoptosis of HUVECs was inhibited while survival, migration and angiogenesis were improved by co-culture of hMSCs and HUVECs. The underlying mechanism may be that hMSCs could secrete a number of cytokines and improve niche, which might be helpful in the treatment of femoral head necrosis.