Fluoroquinolone Resistance in Clinical Isolates of Streptococcus pneumoniae from Asian Countries: ANSORP Study

• Published in: Microbial drug resistance (Larchmont, N.Y.) Vol. 10; no. 1; pp. 37 - 42
• Main Authors: Oh, Won Sup, Suh, Ji Yoeun, Song, Jae-Hoon, Ko, Kwan Soo, Jung, Sook-In, Peck, Kyong Ran, Lee, Nam Yong, Yang, Yonghong, Chongthaleong, Anan, Chiu, Cheng-Hsun, Kamarulzaman, Adeeba, Parasakthi, Navartnam, Lalitha, M.K., Perera, Jennifer, Yee, Ti Teow, Kumarasinghe, Gamini, Carlos, Celia C.
• Format: Journal Article
• Language: English
• Published: United States Mary Ann Liebert, Inc 01.03.2004
• Subjects: Amino acids; Anti-Bacterial Agents - pharmacology; Asia - epidemiology; Bacteria; Ciprofloxacin - pharmacology; Deoxyribonucleic acid; DNA; DNA Gyrase - genetics; DNA Topoisomerase IV - genetics; DNA, Bacterial - genetics; Drug Resistance, Bacterial; Drug therapy; Electrophoresis; Electrophoresis, Gel, Pulsed-Field; Epidemiology; Fluoroquinolones - pharmacology; Microbial Sensitivity Tests; Mutation; Pneumococcal Infections - epidemiology; Pneumococcal Infections - microbiology; Reverse Transcriptase Polymerase Chain Reaction; Serotyping; Streptococcus pneumoniae; Streptococcus pneumoniae - drug effects; Asia
• ISSN: 1076-6294; 1931-8448
• DOI: 10.1089/107662904323047781

Seventeen clinical isolates of Streptococcus pneumoniae showing reduced susceptibility to ciprofloxacin (MIC ≥ 4 μ g/ml) collected from eight different Asian countries were analyzed by antimicrobial susceptibility, serotyping, pulsed-field gel electrophoresis (PFGE), and DNA sequencing of the quinolone resistance-determining regions (QRDRs) in gyrA, gyrB, parC , and parE . All isolates but one showed more than one amino acid alteration in QRDRs of four responsible genes. Ile460 → Val in parE was the most common mutation. Data suggest that Lys137 → Asn in parC may be a primary step in the development of high-level and multiple FQ resistance. An additional mutation of Ser81 → Phe in gyrA resulted in high-level resistance to ciprofloxacin, levofloxacin, and gatifloxacin, whereas Ser79 → Phe in par C may exert an important role in the development of moxifloxacin resistance. Two novel amino acid changes in gyrB , Ala390 → Val and Asn423 → Thr, were found. Data from PFGE suggest an introduction and local spread of multiple resistant Spain 23F -1 clone in Hong Kong, but isolates from other Asian countries were not related to this clone.