Label-free detection of low-molecular-weight samples using a terahertz chemical microscope

A terahertz chemical microscope (TCM) has been proposed and developed to visualize the distribution of biomolecular interactions on a sensing plate without any labels. In this study, the concanavalin A (Con A)-d-(+)-mannose (mannose) interaction was detected using the TCM with mannose applied as the...

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Bibliographic Details
Published inApplied physics express Vol. 9; no. 4; pp. 42401 - 42404
Main Authors Kuwana, Takuya, Ogawa, Masahiro, Sakai, Kenji, Kiwa, Toshihiko, Tsukada, Keiji
Format Journal Article
LanguageEnglish
Published The Japan Society of Applied Physics 01.04.2016
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Summary:A terahertz chemical microscope (TCM) has been proposed and developed to visualize the distribution of biomolecular interactions on a sensing plate without any labels. In this study, the concanavalin A (Con A)-d-(+)-mannose (mannose) interaction was detected using the TCM with mannose applied as the analyte and Con A immobilized on the sensing plate. To demonstrate this interaction, the amplitude of terahertz pulses as a function of Con A-mannose interaction time, as well as the Con A-mannose coupling concentration, was evaluated. The results suggest that coupling kinetics may be evaluated using a TCM.
ISSN:1882-0778
1882-0786
DOI:10.7567/APEX.9.042401