Determination of Vitamins A and E Exploiting Cloud Point Extraction and Micellar Liquid Chromatography

• Published in: Analytical letters Vol. 44; no. 5; pp. 778 - 786
• Main Authors: Ortega, Ana Claudia, da Silva, Danielle Cristina, Visentainer, Jesuí V., de Souza, Nilson Evelázio, de Cinque Almeida, Vitor, Oliveira, Cláudio C.
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 15.03.2011; Taylor & Francis
• Subjects: Analytical chemistry; Buffer solutions; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography; Cloud point extraction; Clouds; Exact sciences and technology; Extraction; HPLC; Mathematical analysis; Other chromatographic methods; Phosphates; Triton; Vitamins; Vitamins A and E; Phosphates; Cloud point extraction; Ascorbic acid; Extraction process; Vitamin; HPLC chromatography; Tungsten V; Alcohol; Liquid chromatography; Sodium compound; Method; Mobile phase; Buffer solution; Retention time; Lauryl sulfate; Cloud point; Sample preparation; Detection limit; pH; Extraction; HPLC; Stationary phase; Vitamins A and E
• ISSN: 0003-2719; 1532-236X
• DOI: 10.1080/00032711003783184

Cloud point extraction and micellar chromatographic methods were developed for determination of vitamins A and E. The stationary phase was C18 and the mobile phase was 3.00% (w/v) SDS, 15.0% (v/v) butyl alcohol and 0.02 mol L −1 phosphate buffer solution at pH 7.0. The retention times for vitamins A and E were 9.6 and 13.0, respectively. The extraction solution was 100 mmol L −1 Triton X-100, 650 mg NaCl and 1.0% ascorbic acid at 70°C for 30 min. The method is precise (r.s.d. < 7%), the linear range was from 5.0 up to 360.0 mg L −1 for both vitamins. Recovery test showed recuperation between 90.2 and 99.2%, and LOD and LOQ of 0.234 and 0.108 mg L −1 , 0.780 and 0.360 mg L −1 to vitamins A and E were found.