Physiological function of NbRanBP1 in Nicotiana benthamiana

• Published in: Molecules and cells Vol. 26; no. 3; pp. 270 - 277
• Main Authors: Cho, H.K. (Yonsei University, Seoul, Republic of Korea), Park, J.A. (Yonsei University, Seoul, Republic of Korea), Pai, H.S. (Yonsei University, Seoul, Republic of Korea), E-mail: hspai@yonsei.ac.kr
• Format: Journal Article
• Language: English
• Published: United States 한국분자세포생물학회 30.09.2008
• Subjects: Amino Acid Sequence; Animals; bimolecular fluorescence complementation; Cation Transport Proteins - genetics; Cation Transport Proteins - metabolism; Cell Nucleus - metabolism; GENE; Gene Expression Regulation, Plant; Gene Silencing; GENES; Membrane Potential, Mitochondrial - physiology; Molecular Sequence Data; Nicotiana - genetics; Nicotiana - metabolism; nuclear envelope; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Phenotype; Plant Leaves - anatomy & histology; Plant Leaves - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Reactive Oxygen Species - metabolism; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Sequence Alignment; stress symptoms; virus-induced gene silencing; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1016/S1016-8478(23)13995-1

This study addresses the physiological functions of the Ran-binding protein homolog NbRanBP1 in Nicotiana benthamiana. Virus-induced gene silencing (VIGS) of NbRanBP1 caused stunted growth, leaf yellowing, and abnormal leaf morphology. The NbRanBP1 gene was constitutively expressed in diverse tissues and an NbRanBP1:GFP fusion protein was primarily localized to the nuclear rim and the cytosol. BiFC analysis revealed in vivo interaction between NbRanBP1 and NbRan1 in the nuclear envelope and the cytosol. Depletion of NbRanBP1 or NbRan1 reduced nuclear accumulation of a NbBTF3:GFP marker protein. In the later stages of development, NbRanBP1 VIGS plants showed stress responses such as reduced mitochondrial membrane potential, excessive production of reactive oxygen species, and induction of defense-related genes. The molecular role of RanBP1 in plants is discussed in comparison with RanBP1 function in yeast and mammals.