Growth-promoting effects of Aspergillus Elegans and the dark septate endophyte (DSE) Periconia macrospinosa on cucumber

• Published in: Archives of microbiology Vol. 206; no. 5; p. 226
• Main Authors: Sidhoum, Warda, Dib, Soulef, Alim, Yousra, Anseur, Sarra, Benlatreche, Sabrina, Belaidouni, Zoulikha Mouna, Chamouma, Fatiha El Zahra
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2024; Springer Nature B.V
• Subjects: Aspergillus; Biochemistry; Biofertilizers; Biomedical and Life Sciences; Biotechnology; Cell Biology; Chlorophyll; Cucumbers; Cucumis sativus; Ecology; Endophytes; Food plants; Food security; Fungi; Fusarium oxysporum; growth promotion; Host plants; Hydrogen cyanide; Hydrogen production; Life Sciences; Microbial Ecology; Microbiology; nutrition; Original Paper; Periconia; phosphates; Phylogeny; Phytopathogenic fungi; Plant growth; Plant roots; Plant stress; Salinity tolerance; Salt tolerance; Sodium chloride; Solubilization; species; stress tolerance; Sustainable agriculture; Sustainable production; Vegetables; Verticillium dahliae; Wild plants; zinc; Endophytic fungi; Plant growth; Cucumber; Plant growth promoting fungi; Periconia macrospinosa; Aspergillus Elegans
• ISSN: 0302-8933; 1432-072X; 1432-072X
• DOI: 10.1007/s00203-024-03958-w

Cucurbits are subject to a variety of stresses that limit their sustainable production, despite their important role in ensuring food security and nutrition. Plant stress tolerance can be enhanced through fungal endophytes. In this study, two endophytes isolated from wild plant roots, were tested to determine their effect on the growth promotion of cucumber ( Cucumis sativus L.) plants. The phylogenetic analysis revealed that the designated isolates were Aspergillus elegans and Periconia macrospinosa . The results of the Plant Growth Promoting Fungal (PGPF) tests showed that both Aspergillus elegans and Periconia macrospinosa have a zinc solubilizing capacity, especially A. elegans, with a solubilization index higher than 80%. Also, both have a high salt tolerance (10–15% NaCl for P. macrospinosa and A. elegans , respectively), cellulolytic activity, and inhibition indices of 40–64.53%. A. elegans and P. macrospinosa had antagonistic effects against the cucumber phytopathogenic fungi Verticillium dahliae and Fusarium oxysporum , respectively. However, A. elegans and P. macrospinosa didn’t exhibit certain potential plant benefits, such as the production of hydrogen cyanide (HCN) and phosphate solubilization. The chlorophyll content and growth parameters of two-month-old cucumber plants inoculated with the fungal species were significantly better than those of the controls (non-inoculated); the shoot dry weights of inoculated plants were increased by 138% and 170% for A. elegans and P. macrospinosa , respectively; and the root colonization by fungal endophytes has also been demonstrated. In addition to the fact that P. macrospinosa has long been known as PGPF, this is the first time that the ability of A. elegans to modulate host plant growth has been demonstrated, with the potential to be used as a biofertilizer in sustainable agriculture.