Vibrio nitrifigilis sp. nov., a marine nitrogen-fixing bacterium isolated from the lagoon sediment of an islet inside an atoll

• Published in: Antonie van Leeuwenhoek Vol. 114; no. 7; pp. 933 - 945
• Main Authors: Huang, Wei-Sheng, Wang, Li-Ting, Chen, Jwo-Sheng, Chen, Yen-Ting, Wei, Sean Ting-Shyang, Chiang, Yin-Ru, Wang, Pei-Ling, Lee, Tzong-Huei, Lin, Shih-Ting, Huang, Lina, Shieh, Wung Yang
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.07.2021; Springer Nature B.V
• Subjects: Ammonium chloride; Anaerobic conditions; aquatic bacteria; Atolls; Bacteria; Biomedical and Life Sciences; Carbohydrates; Deoxyribonucleic acid; DNA; DNA sequencing; Fatty acids; Genes; genomics; Glucose; Lagoons; Life Sciences; Lipids; Medical Microbiology; Microbiology; New species; Nitrogen; Nitrogen fixation; nitrogen-fixing bacteria; Nitrogenation; Nucleotide sequence; Original Paper; phenotype; Phosphatidylethanolamine; phosphatidylethanolamines; Phosphatidylserine; phosphatidylserines; Phylogeny; Plant Sciences; rRNA 16S; sediments; Sodium chloride; Soil Science & Conservation; Substrates; Taiwan; Vibrio; Taiwan; Multilocus sequence analysis (MLSA); Nitrogen-fixing bacteria; Marine bacteria; Vibrionaceae; Vibrio nitrifigilis; Vibrio
• ISSN: 0003-6072; 1572-9699; 1572-9699
• DOI: 10.1007/s10482-021-01567-x

A nitrogen-fixing isolate of facultatively anaerobic, marine bacterium, designated strain NFV-1 T , was recovered from the lagoon sediment of Dongsha Island, Taiwan. It was a Gram-negative rod which exhibited motility with monotrichous flagellation in broth cultures. The strain required NaCl for growth and grew optimally at about 25–35 °C, 3% NaCl and pH 7–8. It grew aerobically and could achieve anaerobic growth by fermenting d -glucose or other carbohydrates as substrates. NH 4 Cl could serve as a sole nitrogen source for growth aerobically and anaerobically, whereas growth with N 2 as the sole nitrogen source was observed only under anaerobic conditions. Cellular fatty acids were predominated by C 16:1 ω 7 c , C 16:0 , and C 18:1 ω 7 c . The major polar lipids consisted of phosphatidylethanolamine and phosphatidylserine. Strain NFV-1 T had a DNA G + C content of 42.5 mol%, as evaluated according to the chromosomal DNA sequencing data. Analyses of sequence similarities and phylogeny based on the 16S rRNA genes, together with the housekeeping genes, gyrB , ftsZ , mreB , topA and gapA , indicated that the strain formed a distinct species-level lineage in the genus Vibrio of the family Vibrionaceae . These phylogenetic data and those from genomic and phenotypic characterisations support the establishment of a novel Vibrio species, for which the name Vibrio nitrifigilis sp. nov. (type strain NFV-1 T  = BCRC 81211 T  = JCM 33628 T ) is proposed.