Rice OsBT1 regulates seed dormancy through the glycometabolism pathway

• Published in: Plant physiology and biochemistry Vol. 151; pp. 469 - 476
• Main Authors: Song, Weihan, Hao, Qixian, Cai, Mengying, Wang, Yihua, Zhu, Xingjie, Liu, Xi, Huang, Yunshuai, Nguyen, Thanhliem, Yang, Chunyan, Yu, Jiangfeng, Wu, Hongming, Chen, Liangming, Tian, Yunlu, Jiang, Ling, Wan, Jianmin
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.06.2020
• Subjects: Germination; Glycometabolism; Oryza sativa; OsBT1; Glycometabolism; ABA; Germination; QTLs; InDels; Oryza sativa; MCF; HAI; TCA; Nip; PP2C; OsBT1; SNPs; PHS; WT; DPH; QRT-PCR
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2020.03.055

Seed dormancy and germination in rice (Oryza sativa L.) are complex and important agronomic traits that involve a number of physiological processes and energy. A mutant named h470 selected from a60Co-radiated indica cultivar N22 population had weakened dormancy that was insensitive to Gibberellin (GA) and Abscisic acid (ABA). The levels of GA4 and ABA were higher in h470 than in wild-type (WT) plants. The gene controlling seed dormancy in h470 was cloned by mut-map and transgenesis and confirmed to encode an ADP-glucose transporter protein. A 1 bp deletion in Os02g0202400 (OsBT1) caused the weaker seed dormancy in h470. Metabolomics analyses showed that most sugar components were higher in h470 seeds than the wild type. The mutation in h470 affected glycometabolism. •We identified a new allele of OsBT1 regulating seed dormancy in rice.•The germination phenotype of h470 was not regulated by GA or ABA.•The OsBT1 protein regulates seed dormancy through the glycometabolism pathway.