Relation of atrophic gastritis with Helicobacter pylori-CagA(+) and interleukin-1 gene polymorphisms

• Published in: World journal of gastroenterology : WJG Vol. 14; no. 42; pp. 6481 - 6487
• Main Authors: Sierra, Rafaela, Une, Clas, Ramirez, Vanessa, Alpizar-Alpizar, Warner, Gonzalez, Maria-I, Ramirez, Jose-A, De Mascarel, Antoine, Cuenca, Patricia, Perez-Perez, Guillermo, Megraud, Francis
• Format: Journal Article
• Language: English
• Published: United States Institute of Health Research,University of Costa Pica,San Jose 2060,Costa Pica%Department of Statistics,University of Costa Pica,San Jose 2060,Costa Pica%Department of Pathology,Calderón Guardia Hospital,Costa Pica,San José 2060,Costa Pica%University Victor Segalen Bordeaux 2,146,rue Leo-Saignat,Case 76,Bordeaux Cedex 33076,France%Departments of Medicine and Microbiology,New York University School of Medicine,NYU.Langone Medical Center,550 First Avenue,New York 10016,United States 14.11.2008; The WJG Press and Baishideng
• Subjects: Alcohol Drinking - adverse effects; Antigens, Bacterial - genetics; Atrophy; Bacterial Proteins - genetics; Biopsy; Costa Rica; Diet - adverse effects; Dyspepsia - genetics; Dyspepsia - immunology; Dyspepsia - microbiology; Female; Gastritis - genetics; Gastritis - immunology; Gastritis - microbiology; Gastritis - pathology; Gastroscopy; Genetic Predisposition to Disease; Helicobacter Infections - complications; Helicobacter Infections - genetics; Helicobacter Infections - immunology; Helicobacter Infections - microbiology; Helicobacter Pylori; Helicobacter pylori - genetics; Humans; Interleukin 1 Receptor Antagonist Protein - genetics; Interleukin-1beta - genetics; Logistic Models; Male; Middle Aged; Odds Ratio; Pepsinogen A - blood; Pepsinogen C - blood; Peptic Ulcer - genetics; Peptic Ulcer - immunology; Peptic Ulcer - microbiology; Polymorphism, Genetic; Risk Assessment; Risk Factors; Smoking - adverse effects; Surveys and Questionnaires; Costa Rica; Pepticulcers; Interleukins; Pepsinogen; Atrophic gastritis; Helicobacter pylori-CagA
• ISSN: 1007-9327
• DOI: 10.3748/wjg.14.6481

To determine the association of Helicobacter pylori (H pylori) CagA(+) infection and pro-inflammatory polymorphisms of the genes interleukin (IL)-1RN and IL-1B with the risk of gastric atrophy and peptic ulcers in a dyspeptic population in Costa Rica, a country with high incidence and mortality of gastric cancer. Seven biopsy specimens, a fasting blood sample and a questionnaire concerning nutritional and sociodemographic factors were obtained from 501 consecutive patients who had undergone endoscopy for dyspeptic symptoms. A histopathological diagnosis was made. Pepsinogen concentrations were analyzed by enzyme linked immunosorbent assay (ELISA). Infection with H pylori CagA(+) was determined by serology and polymerase chain reaction (PCR). IL-1B and IL-1RN polymorphisms genotyping was performed by PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR respectively. In this dyspeptic population, 86% were H pylori positive and of these, 67.8% were positive for CagA. Atrophic antral gastritis (AAG) was associated with CagA(+) status [odd ratio (OR) = 4.1; P < 0.000] and fruit consumption (OR = 0.3; P < 0.00). Atrophic body gastritis (ABG) was associated with pepsinogen PGI/PGII < 3.4 (OR = 4.9; P < 0.04) and alcohol consumption (OR = 7.3; P < 0.02). Duodenal ulcer was associated with CagA(+) (OR = 2.9; P < 0.04) and smoking (OR = 2.4; P < 0.04). PGI < 60 microg/L as well as PGI/PGII < 3.4 were associated with CagA(+). In a dyspeptic population in Costa Rica, H pylori CagA(+) is not associated with ABG, but it is a risk factor for AAG. The pro-inflammatory cytokine polymorphisms IL-1B + 3945 and IL-1RN are not associated with the atrophic lesions of this dyspeptic population.