Protective effects of Rheum tanguticum polysaccharide against hydrogen peroxide-induced intestinal epithelial cell injury

• Published in: World journal of gastroenterology : WJG Vol. 11; no. 10; pp. 1503 - 1507
• Main Authors: Liu, Lin-Na, Mei, Qi-Bing, Liu, Li, Zhang, Feng, Liu, Zhen-Guo, Wang, Zhi-Peng, Wang, Ru-Tao
• Format: Journal Article
• Language: English
• Published: United States Department of Pharmacology,Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China 14.03.2005; Baishideng Publishing Group Inc
• Subjects: Basic Research; Cell Line; Humans; Hydrogen Peroxide - pharmacology; Intestinal Mucosa - drug effects; Intestinal Mucosa - pathology; Oxidants - pharmacology; Oxidative Stress - drug effects; Plant Extracts - pharmacology; Polysaccharides - pharmacology; Protective Agents - pharmacology; Rheum - chemistry; 保护机制; 肠道上皮细胞; 肠道损伤; 过氧化氢; Catalase; Rheum tanguticum polysaccharide; Intestinal epithelial cells; Apoptosis; Necrosis
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v11.i10.1503

AIM: To describe the effect of Rheum tanguticum polysaccharide (RTP) on hydrogen peroxide-induced human intestinal epithelial cell injury.METHODS: Hydrogen peroxide (100 μmol/L) was introduced to induce human intestinal epithelial cell injury.Cells were pretreated with RTP (30,100,300 μg/mL) for 24 h before exposure to hydrogen peroxide. Cell viability was detected by MTT assay and morphological observation.Acridine orange staining and flow cytometry were performed to assess cell apoptosis. Lactate dehydrogenase (LDH) activity, production of malondialdehyde (MDA) and superoxide dismutase (SOD) activity were measured by spectrophotometry with corresponding assay kits.RESULTS: Following exposure to H2O2, a marked decrease in cell survival and SOD activity, increased production of MDA, LDH leakage and cell apoptosis were found.Pretreatment of the cells with RTP could significantly elevate cell survival, SOD activity and decrease the level of MDA, LDH activity and cell apoptosis.CONCLUSION: RTP may have cytoprotective and anti-oxidant effects against H2O2. induced intestinal epithelial cell injury by inhibiting cell apoptosis and necrosis. This might be one of the possible mechanisms of RTP for the treatment of ulcerative colitis in rats.