Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis

To find potential targets of novel antimicrobial agents, we identified essential genes of Streptococcus pneumoniae using comparative genomics and allelic replacement mutagenesis. We compared the genome of S. pneumoniae R6 with those of Bacillus subtilis, Enterococcus faecalis, Escherichia coli, and...

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Bibliographic Details
Published inMolecules and cells Vol. 19; no. 3; pp. 365 - 374
Main Authors Song, J.H. (Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul, Republic of Korea), E-mail: jhsong@smc.samsung.co.kr, Oh, W.S. (Sungkyunkwan University School of Medicine, Seoul, Republic of Korea), Yoon, H.S. (LG Life Sciences, Inc., Daejeon, Republic of Korea), Jeong, J.Y. (Asan Institute for Life Sciences, Seoul, Republic of Korea), Chun, J.S. (Seoul National University, Seoul, Republic of Korea), Ko, K.S. (Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul, Republic of Korea), Lee, J.Y. (Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul, Republic of Korea), Baek, J.Y. (Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul, Republic of Korea)
Format Journal Article
LanguageEnglish
Published United States 한국분자세포생물학회 30.06.2005
Subjects
Allelic Replacement Mutagenesis
Essential Genes
Genes, Bacterial
Genes, Essential
Genome, Bacterial
Genomics
MUTACION
Mutagenesis, Insertional - methods
MUTATION
New Antimicrobial Agent
Pneumococci
Polymerase Chain Reaction - methods
Streptococcus pneumoniae - genetics
생물학
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Summary:To find potential targets of novel antimicrobial agents, we identified essential genes of Streptococcus pneumoniae using comparative genomics and allelic replacement mutagenesis. We compared the genome of S. pneumoniae R6 with those of Bacillus subtilis, Enterococcus faecalis, Escherichia coli, and Staphylococcus aureus, and selected 693 candidate target genes with greater than 40% amino acid sequence identity to the corresponding genes in at least two of the other species. The 693 genes were disrupted and 133 were found to be essential for growth. Of these, 32 encoded proteins of unknown function, and we were able to identify orthologues of 22 of these genes by genomic comparisons.
Bibliography:T10
2006013381
G704-000079.2005.19.3.003
ISSN:1016-8478
0219-1032
DOI:10.1016/S1016-8478(23)13181-5