Renoprotective effect of tectorigenin glycosides isolated from Iris spuria L. (Zeal) against hyperoxaluria and hyperglycemia in NRK-49Fcells

• Published in: Natural product research Vol. 35; no. 6; pp. 1029 - 1034
• Main Authors: Divya, Ganesan, Albert, Abhishek, Singab, Abdel Nasser B., Ayoub, Iriny M., Al-Sayed, Eman, Paul, Eldho, Manoharan, Kumarriah, Saso, Luciano, Selvam, Govindan Sadasivam
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 19.03.2021; Taylor & Francis Ltd
• Subjects: Animals; antioxidant; Antioxidants; Antioxidants - pharmacology; Aquatic plants; Beta cells; Calculi; Cell Death - drug effects; Cell Line; Cell Survival - drug effects; Cytoprotection - drug effects; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Enzymatic activity; Fibrosis; Gene expression; Gene Expression Regulation - drug effects; Genes; Glucose; Glucose - toxicity; Glucose tolerance; Glucosides; Glycosides; Glycosides - chemistry; Glycosides - isolation & purification; Glycosides - pharmacology; Glycosides - therapeutic use; Hyperglycemia; Hyperglycemia - drug therapy; Hyperglycemia - genetics; Hyperoxaluria; Hyperoxaluria - drug therapy; Hyperoxaluria - genetics; Insulin; Insulin resistance; Iris Plant - chemistry; Iris spuria; Iris spuria L; Isoflavones - chemistry; Isoflavones - pharmacology; Isoflavones - therapeutic use; Kidney - pathology; Kidney stones; Nephrolithiasis; oxalate; Oxalic acid; Oxidative stress; Oxidative Stress - drug effects; Oxidative Stress - genetics; Protective Agents - chemistry; Protective Agents - pharmacology; Protective Agents - therapeutic use; Rats; Reactive Oxygen Species - metabolism; Rhizomes; tectorigenin derivatives; hyperglycemia; antioxidant; Iris spuria L; oxalate; tectorigenin derivatives; hyperoxaluria
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2019.1613396

Oxidative stress has been identified as an underlying factor in the development of insulin resistance, β-cell dysfunction, impaired glucose tolerance and type 2 diabetes mellitus and it also play major role in kidney stone formation. The present study is aimed to elucidate the in vitro nephroprotective activity of two isoflavonoid glycosides, tectorigenin 7-O-β-D-glucosyl-(1→6)-β-D-glucoside (1) and tectorigenin 7-O-β-D-glucosyl-4′-O-β-D-glucoside (2) isolated from the n-BuOH fraction of Iris spuria L. (Zeal) rhizome MeOH extract against oxalate and high glucose-induced oxidative stress in NRK-49F cells. The results revealed that compounds 1 and 2 significantly increased the antioxidant enzyme activities and decreased MDA levels in both oxalate and high glucose stress. Treatment with these phytochemicals effectively down-regulated expression of crystal modulator genes and pro-fibrotic genes in oxalate and high glucose-mediated stress respectively. This study indicates cytoprotective, antioxidant, anti-urolithic and anti-diabetic effects of compounds 1 and 2 against oxalate and high glucose stress.