Shewanella jiangmenensis sp. nov., isolated from aquaculture water

• Published in: Archives of microbiology Vol. 204; no. 3; p. 198
• Main Authors: Song, Yingli, Li, Anzhang, Xu, Shuaishuai, Zhang, Mingxia, Yao, Qing, Ma, Lian, Zhu, Honghui
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.03.2022; Springer Nature B.V
• Subjects: Antibiotics; Aquaculture; Archives & records; Bacterial Typing Techniques; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Chromatography; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Drug resistance; Ecology; Farming; Fatty acids; Fatty Acids - chemistry; Gene sequencing; Genes; Genomes; Hybridization; Laboratories; Life Sciences; Lipids; Litopenaeus vannamei; Microbial Ecology; Microbiology; Microscopy; New species; nucleic acid hybridization; Nucleotides; Original Paper; Phosphatidylethanolamine; phosphatidylethanolamines; Phosphatidylglycerol; Phylogenetics; Phylogeny; Physiology; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Shellfish; Shewanella; Shewanella - genetics; Shewanella amazonensis; Sodium chloride; Software; Water; Genotypes and phenotypes; Aquaculture water; Polyphasic taxonomy; Genomic analysis; Shewanella jiangmenensis
• ISSN: 0302-8933; 1432-072X; 1432-072X
• DOI: 10.1007/s00203-022-02761-9

A Gram-stain-negative and facultatively anaerobic bacterial strain designated as JM162201 T was isolated from aquaculture water for farming Pacific white shrimp ( Litopenaeus vannamei ). The genome size of strain JM162201 T was 4,436,316 bp, and the genomic DNA G + C content was 55.0%. Phylogenetic analysis based on 16S rRNA gene sequences and genomes showed that strain JM162201 T belonged to the genus Shewanella and was closely related to Shewanella litorisediminis SMK1-12 T (97.1%), Shewanella khirikhana TH2012 T (97.0%), and Shewanella amazonensis SB2B T (96.0%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain JM162201 T and three reference type strains were below the recognized thresholds of 95.0–96.0% (for ANI) and 70.0% (for dDDH) for species delineation. Growth occurred at 10–40 °C (optimum, 30 °C), at pH 4.0–10.0 (optimum, 7.0–8.0), and in 0–6.0% NaCl (w/v, optimum, 0–0.1%). The major cellular fatty acids of strain JM162201 T were summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ), C 17:1 ω 8 c , iso-C 15:0 , C 16:0 , and C 15:0 . The predominant quinones were MK7, Q-7, and Q-8. The major polar lipids were phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). Based on the polyphasic taxonomical analyses, strain JM162201 T represents a novel species of the genus Shewanella , for which the name Shewanella jiangmenensis sp. nov. is proposed, with the type strain JM162201 T (= GDMCC 1.2006 T  = KCTC 82340 T ).