IS1500, an IS3-like element from Leptospira interrogans

• Published in: Microbiology (Society for General Microbiology) Vol. 141; no. 9; pp. 2165 - 2173
• Main Authors: Boursaux-Eude, Caroline, Girons, Isabelle Saint, Zuerner, Richard
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.09.1995; Society for General Microbiology
• Subjects: Amino Acid Sequence; Bacterial Proteins - genetics; Bacteriology; Base Sequence; Biological and medical sciences; DNA Nucleotidyltransferases - genetics; DNA Transposable Elements; Fundamental and applied biological sciences. Psychology; Genetics; Leptospira interrogans - genetics; Microbiology; Molecular Sequence Data; Open Reading Frames; Polymerase Chain Reaction; Repetitive Sequences, Nucleic Acid; Sequence Alignment; Transposases; Chromosomal aberration; Genetic mapping; Genome organization; Spirochaetales; Nucleotide sequence; Chromosome insertion; Copy number; Transposable element; IS element; Leptospiraceae; Restriction map; Bacteria; Abnormal chromosome; Leptospira interrogans; Aminoacid sequence
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/13500872-141-9-2165

Unité de Bacériologie Moléculaire et Médicale, Institut Pasteur, 75724, Paris Cedex 15, France Leptospirosis and Mycobacteriosis Research Unit, National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, PO Box 70, Ames, IA 50010, USA ABSTRACT Copies of an insertion-sequence (IS)-like element were isolated from two closely related serovars of Leptospira interrogans sensu stricto. Nucleotide sequence analysis of the 1236 bp element showed a characteristic IS structure with terminal imperfect inverted repeats (IRs) flanking a 1159 bp central region. This element was designated IS 1500. Four open reading frames ( orfA-orfD ) were found in the central ‘unique’ region of IS 1500. Similarities were detected between ORFA and ORFB and the putative transposases from members of the IS3 family of transposable elements. IS 1500 or IS 1500-like sequences were also detected in all other pathogenic leptospiral serovars, but not in the saprophytic species L. biflexa. Differences in IS1500 copy numbers in members of the same species suggest that this element can transpose. Physical mapping of IS 1500 insertions in L. interrogans serovars icterohaemorrhagiae and pomona showed insertions were only on the large chromosomal replicon. The location of some IS1500 insertions coincides with regions of the genome that have undergone large rearrangements. Tel: +1 515 239 8392. Fax: +1 515 239 8458 e-mail: rzuerner@asrr.arsusda.gov Keywords: insertion sequence, Leptospira , IS3 -like